Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22647 | 3' | -54.1 | NC_005091.1 | + | 51229 | 0.66 | 0.790008 |
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Target: 5'- cCGUCAaUCGCCGCGCgucgcugUGGGaagcaacGAUGu -3' miRNA: 3'- -GUAGU-AGCGGCGUGaa-----ACCCgc-----CUAC- -5' |
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| 22647 | 3' | -54.1 | NC_005091.1 | + | 3939 | 0.66 | 0.790008 |
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Target: 5'- gAUCGUCGUCGCcauccGGGUcgGGAUGa -3' miRNA: 3'- gUAGUAGCGGCGugaaaCCCG--CCUAC- -5' |
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| 22647 | 3' | -54.1 | NC_005091.1 | + | 38125 | 0.66 | 0.780014 |
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Target: 5'- aAUCAUCGUCGCAU----GGUGGAc- -3' miRNA: 3'- gUAGUAGCGGCGUGaaacCCGCCUac -5' |
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| 22647 | 3' | -54.1 | NC_005091.1 | + | 51553 | 0.69 | 0.595656 |
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Target: 5'- gAUCcgCGCgGCAUcgccaagUGGGCGGAa- -3' miRNA: 3'- gUAGuaGCGgCGUGaa-----ACCCGCCUac -5' |
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| 22647 | 3' | -54.1 | NC_005091.1 | + | 4002 | 0.69 | 0.573584 |
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Target: 5'- aAUCGuuUCGCCGCcCUUcgGGGCGGc-- -3' miRNA: 3'- gUAGU--AGCGGCGuGAAa-CCCGCCuac -5' |
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| 22647 | 3' | -54.1 | NC_005091.1 | + | 10045 | 0.69 | 0.573584 |
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Target: 5'- -uUCAUCGCCGgGCgcUGGGagcaGGAg- -3' miRNA: 3'- guAGUAGCGGCgUGaaACCCg---CCUac -5' |
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| 22647 | 3' | -54.1 | NC_005091.1 | + | 21106 | 0.71 | 0.47755 |
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Target: 5'- aGUCG-CGCCGCGCaUaccugcgaccaUGGGCGGGUu -3' miRNA: 3'- gUAGUaGCGGCGUGaA-----------ACCCGCCUAc -5' |
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| 22647 | 3' | -54.1 | NC_005091.1 | + | 26990 | 0.72 | 0.408653 |
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Target: 5'- -uUCAUCGCCuGCGCcgacauguccUUGGcGCGGAUGa -3' miRNA: 3'- guAGUAGCGG-CGUGa---------AACC-CGCCUAC- -5' |
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| 22647 | 3' | -54.1 | NC_005091.1 | + | 51274 | 0.73 | 0.381082 |
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Target: 5'- aAUCGUCGCCGuCGCgcccgUGGGCGauuuucauuGAUGg -3' miRNA: 3'- gUAGUAGCGGC-GUGaa---ACCCGC---------CUAC- -5' |
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| 22647 | 3' | -54.1 | NC_005091.1 | + | 53281 | 1.07 | 0.001781 |
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Target: 5'- uCAUCAUCGCCGCACUUUGGGCGGAUGc -3' miRNA: 3'- -GUAGUAGCGGCGUGAAACCCGCCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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