Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2266 | 5' | -56.8 | NC_001405.1 | + | 24043 | 0.66 | 0.514549 |
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Target: 5'- cCCgacuggCCAUuUCCUUCUCCUAUagGCAGAa -3' miRNA: 3'- -GGa-----GGUAcGGGAAGAGGGUG--CGUCUg -5' |
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| 2266 | 5' | -56.8 | NC_001405.1 | + | 3777 | 0.66 | 0.514549 |
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Target: 5'- gCCUCCGccGCCgCUUCagCCGCuGCAGcCa -3' miRNA: 3'- -GGAGGUa-CGG-GAAGagGGUG-CGUCuG- -5' |
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| 2266 | 5' | -56.8 | NC_001405.1 | + | 8344 | 0.66 | 0.482763 |
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Target: 5'- -gUCCAUGgUCUggagCUCCCGCGgcgacagguCAGGCg -3' miRNA: 3'- ggAGGUACgGGAa---GAGGGUGC---------GUCUG- -5' |
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| 2266 | 5' | -56.8 | NC_001405.1 | + | 8633 | 0.68 | 0.393756 |
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Target: 5'- aCCUCCGgggGCCCg---CCCGCGUcaccgcuuuuAGAUg -3' miRNA: 3'- -GGAGGUa--CGGGaagaGGGUGCG----------UCUG- -5' |
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| 2266 | 5' | -56.8 | NC_001405.1 | + | 8898 | 0.68 | 0.375464 |
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Target: 5'- aUCUCCGcGUCCggCUCgcuCCACGguGGCg -3' miRNA: 3'- -GGAGGUaCGGGaaGAG---GGUGCguCUG- -5' |
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| 2266 | 5' | -56.8 | NC_001405.1 | + | 13213 | 0.68 | 0.375464 |
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Target: 5'- gCCUCCAggcUGCCCgugucCUCCUgccccaGCGCGcGGCu -3' miRNA: 3'- -GGAGGU---ACGGGaa---GAGGG------UGCGU-CUG- -5' |
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| 2266 | 5' | -56.8 | NC_001405.1 | + | 11392 | 0.68 | 0.366536 |
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Target: 5'- aUCUCCucggGCUCcUCUCCCuCGCGGuCg -3' miRNA: 3'- -GGAGGua--CGGGaAGAGGGuGCGUCuG- -5' |
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| 2266 | 5' | -56.8 | NC_001405.1 | + | 17771 | 0.7 | 0.308213 |
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Target: 5'- gCCggCCAUGCCC--CUCCUACGguG-Ca -3' miRNA: 3'- -GGa-GGUACGGGaaGAGGGUGCguCuG- -5' |
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| 2266 | 5' | -56.8 | NC_001405.1 | + | 25476 | 0.7 | 0.308213 |
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Target: 5'- gCUCCGUGgCCgcgcaCCugGCGGACa -3' miRNA: 3'- gGAGGUACgGGaagagGGugCGUCUG- -5' |
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| 2266 | 5' | -56.8 | NC_001405.1 | + | 23497 | 1.12 | 0.000245 |
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Target: 5'- gCCUCCAUGCCCUUCUCCCACGCAGACa -3' miRNA: 3'- -GGAGGUACGGGAAGAGGGUGCGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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