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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22672 | 3' | -51.8 | NC_005091.1 | + | 23898 | 0.67 | 0.852881 |
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Target: 5'- gAGCAGGUUGuUCGUcUCGGUcaucUGUugGu -3' miRNA: 3'- gUCGUCCAAC-GGCAaGGUCA----ACAugC- -5' |
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| 22672 | 3' | -51.8 | NC_005091.1 | + | 14638 | 0.68 | 0.776716 |
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Target: 5'- aCGGCAGGaaugGUCGgcgUCCAGUUGcaaGCa -3' miRNA: 3'- -GUCGUCCaa--CGGCa--AGGUCAACa--UGc -5' |
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| 22672 | 3' | -51.8 | NC_005091.1 | + | 3716 | 0.68 | 0.766366 |
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Target: 5'- gCAGCAGGUcgGCCGguuUCCAGccaucgggcUUG-ACGa -3' miRNA: 3'- -GUCGUCCAa-CGGCa--AGGUC---------AACaUGC- -5' |
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| 22672 | 3' | -51.8 | NC_005091.1 | + | 47396 | 0.72 | 0.578092 |
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Target: 5'- gCAGCAGGUaugcgGCgGUguuuaUCguGUUGUACGg -3' miRNA: 3'- -GUCGUCCAa----CGgCA-----AGguCAACAUGC- -5' |
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| 22672 | 3' | -51.8 | NC_005091.1 | + | 32975 | 0.72 | 0.544822 |
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Target: 5'- gCAGCGGGUUGCU--UCgAGUUGgccgACGa -3' miRNA: 3'- -GUCGUCCAACGGcaAGgUCAACa---UGC- -5' |
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| 22672 | 3' | -51.8 | NC_005091.1 | + | 14629 | 1.08 | 0.00269 |
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Target: 5'- uCAGCAGGUUGCCGUUCCAGUUGUACGc -3' miRNA: 3'- -GUCGUCCAACGGCAAGGUCAACAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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