Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22680 | 3' | -51.7 | NC_005091.1 | + | 43790 | 0.67 | 0.825011 |
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Target: 5'- aGAGCGCgCCGAGCgcauAGAGCa---UGGCu -3' miRNA: 3'- -UUUGUGaGGCUCG----UCUCGaacaACCG- -5' |
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| 22680 | 3' | -51.7 | NC_005091.1 | + | 7325 | 0.68 | 0.785407 |
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Target: 5'- uGACgACUUCGAGCacggAGAGCg---UGGCg -3' miRNA: 3'- uUUG-UGAGGCUCG----UCUCGaacaACCG- -5' |
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| 22680 | 3' | -51.7 | NC_005091.1 | + | 6193 | 0.68 | 0.775045 |
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Target: 5'- ---gGCUCCuAGCgcgGGGGCUUGcgGGCg -3' miRNA: 3'- uuugUGAGGcUCG---UCUCGAACaaCCG- -5' |
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| 22680 | 3' | -51.7 | NC_005091.1 | + | 55407 | 0.68 | 0.764523 |
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Target: 5'- --cCACUUCGAGCGcGAGCacGcgGGCg -3' miRNA: 3'- uuuGUGAGGCUCGU-CUCGaaCaaCCG- -5' |
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| 22680 | 3' | -51.7 | NC_005091.1 | + | 35113 | 0.7 | 0.664748 |
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Target: 5'- -cACGCgggCCGAGCGGccggucagcAGCUUG-UGGUa -3' miRNA: 3'- uuUGUGa--GGCUCGUC---------UCGAACaACCG- -5' |
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| 22680 | 3' | -51.7 | NC_005091.1 | + | 18603 | 1.1 | 0.001686 |
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Target: 5'- gAAACACUCCGAGCAGAGCUUGUUGGCu -3' miRNA: 3'- -UUUGUGAGGCUCGUCUCGAACAACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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