Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22681 | 3' | -51.8 | NC_005091.1 | + | 55219 | 0.66 | 0.912756 |
Target: 5'- ---cUCGUucGACGc-GGCCCGUGCGc -3' miRNA: 3'- guacAGCGcuUUGCuuUCGGGCACGC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 54258 | 0.66 | 0.897834 |
Target: 5'- cCGUGUCGUcuaAAGCGAAGcGCCCGacacugagcaagGCGg -3' miRNA: 3'- -GUACAGCGc--UUUGCUUU-CGGGCa-----------CGC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 19535 | 0.66 | 0.88463 |
Target: 5'- --cGUCGUGuucGGCGAuuGCUgGUGCa -3' miRNA: 3'- guaCAGCGCu--UUGCUuuCGGgCACGc -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 54678 | 0.66 | 0.883871 |
Target: 5'- cCAUGcuggaaUCGCGcgGCGAAAGCaaaugguCCGUGuCGg -3' miRNA: 3'- -GUAC------AGCGCuuUGCUUUCG-------GGCAC-GC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 26855 | 0.66 | 0.876918 |
Target: 5'- -----gGCGaAGACGcGAGCCgGUGCGg -3' miRNA: 3'- guacagCGC-UUUGCuUUCGGgCACGC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 2875 | 0.67 | 0.843529 |
Target: 5'- ---cUCGCG-AACGgcAGCCCGaGCGc -3' miRNA: 3'- guacAGCGCuUUGCuuUCGGGCaCGC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 940 | 0.67 | 0.843529 |
Target: 5'- --cGUCGCGuacgaccCGGAAGCCguCGUGCc -3' miRNA: 3'- guaCAGCGCuuu----GCUUUCGG--GCACGc -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 24024 | 0.67 | 0.843529 |
Target: 5'- ---uUCGCGuc-CGGGAuGCCCGUGCc -3' miRNA: 3'- guacAGCGCuuuGCUUU-CGGGCACGc -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 41896 | 0.68 | 0.816052 |
Target: 5'- --gGUCGUucAACGgcGGCCgCGUGCa -3' miRNA: 3'- guaCAGCGcuUUGCuuUCGG-GCACGc -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 15160 | 0.68 | 0.816052 |
Target: 5'- --cGUUGCGcccggaucggGGACGAGaccGGCCgCGUGCGu -3' miRNA: 3'- guaCAGCGC----------UUUGCUU---UCGG-GCACGC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 50332 | 0.68 | 0.80648 |
Target: 5'- gCcgGUCGCGAAAauCGAccuGCCCGUaCGu -3' miRNA: 3'- -GuaCAGCGCUUU--GCUuu-CGGGCAcGC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 19791 | 0.68 | 0.78678 |
Target: 5'- cCAUGagCGUGAuGCGAuuGCCgaCGUGCGu -3' miRNA: 3'- -GUACa-GCGCUuUGCUuuCGG--GCACGC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 43538 | 0.68 | 0.78678 |
Target: 5'- --aGUCGUca---GAAAGCCUGUGCGc -3' miRNA: 3'- guaCAGCGcuuugCUUUCGGGCACGC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 34927 | 0.69 | 0.756012 |
Target: 5'- --cGUCGCGGcucucGCGAGAGaCCGUGUc -3' miRNA: 3'- guaCAGCGCUu----UGCUUUCgGGCACGc -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 50561 | 0.7 | 0.713214 |
Target: 5'- --cGUCGUaAGGCGGAuguuGCCgGUGCGg -3' miRNA: 3'- guaCAGCGcUUUGCUUu---CGGgCACGC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 12742 | 0.7 | 0.713214 |
Target: 5'- --cGUCGCGuucauuCGcuGGCCCcGUGCGa -3' miRNA: 3'- guaCAGCGCuuu---GCuuUCGGG-CACGC- -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 54096 | 0.72 | 0.602044 |
Target: 5'- -uUGUCGCGuu-CGGucGCgCCGUGCa -3' miRNA: 3'- guACAGCGCuuuGCUuuCG-GGCACGc -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 51585 | 0.75 | 0.403655 |
Target: 5'- ---cUCGCGAuGCGAuagaacuGGCCCGUGCa -3' miRNA: 3'- guacAGCGCUuUGCUu------UCGGGCACGc -5' |
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22681 | 3' | -51.8 | NC_005091.1 | + | 19280 | 1.1 | 0.002369 |
Target: 5'- gCAUGUCGCGAAACGAAAGCCCGUGCGa -3' miRNA: 3'- -GUACAGCGCUUUGCUUUCGGGCACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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