Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22681 | 5' | -61.6 | NC_005091.1 | + | 17622 | 0.66 | 0.401926 |
Target: 5'- aCGCUCCCG---GCCGCGCaucuUCGCAa-- -3' miRNA: 3'- -GUGAGGGCucgCGGCGCG----AGCGUgua -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 46292 | 0.66 | 0.401926 |
Target: 5'- cCACUCgugccagaguUCGAG-GCCGCGCUCGUg--- -3' miRNA: 3'- -GUGAG----------GGCUCgCGGCGCGAGCGugua -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 47345 | 0.66 | 0.384403 |
Target: 5'- aACUUCaaaGAcGCGCUGcCGCUCGUGCGg -3' miRNA: 3'- gUGAGGg--CU-CGCGGC-GCGAGCGUGUa -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 10147 | 0.66 | 0.374984 |
Target: 5'- gCACgCgCCGAGCGCCGCacGUUCcugauguGCGCGUg -3' miRNA: 3'- -GUGaG-GGCUCGCGGCG--CGAG-------CGUGUA- -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 6725 | 0.66 | 0.367394 |
Target: 5'- aGCgaagCagaCGAGCG-CGCGCUCGgGCAUg -3' miRNA: 3'- gUGa---Gg--GCUCGCgGCGCGAGCgUGUA- -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 30129 | 0.67 | 0.334957 |
Target: 5'- uUACaggCCCGAG-GCCGaCGCuuUCGCGCGg -3' miRNA: 3'- -GUGa--GGGCUCgCGGC-GCG--AGCGUGUa -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 33464 | 0.67 | 0.327182 |
Target: 5'- uCACUuucaaUCUGAGCGCgaaGCGCUCGCuCGg -3' miRNA: 3'- -GUGA-----GGGCUCGCGg--CGCGAGCGuGUa -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 42563 | 0.67 | 0.30981 |
Target: 5'- gCGCUCgCCGAgaagacgcuuguccGCGCCGCGCaCGCuCGg -3' miRNA: 3'- -GUGAG-GGCU--------------CGCGGCGCGaGCGuGUa -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 40957 | 0.68 | 0.290325 |
Target: 5'- cCGCgUCCCGcccAGCGCUGCGUgcgcugCGCGCc- -3' miRNA: 3'- -GUG-AGGGC---UCGCGGCGCGa-----GCGUGua -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 14813 | 0.68 | 0.290325 |
Target: 5'- gACU-CCGGGUGCUacgGCGCUCGCAa-- -3' miRNA: 3'- gUGAgGGCUCGCGG---CGCGAGCGUgua -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 33525 | 0.68 | 0.283356 |
Target: 5'- gACUUCCGAaagagcuucGCGCagCGCGCUCGCGa-- -3' miRNA: 3'- gUGAGGGCU---------CGCG--GCGCGAGCGUgua -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 10894 | 0.68 | 0.279238 |
Target: 5'- uCGCUgCCGAGUGCaaugCGCGCcuuggccgcgaucguUCGCGCAc -3' miRNA: 3'- -GUGAgGGCUCGCG----GCGCG---------------AGCGUGUa -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 17406 | 0.68 | 0.27652 |
Target: 5'- uGCUCgUCG-GCGCUGCGUUCGCcuGCAa -3' miRNA: 3'- gUGAG-GGCuCGCGGCGCGAGCG--UGUa -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 29424 | 0.69 | 0.238251 |
Target: 5'- cCACgaCCGAGaCGCCGCcCUUGCGCGUc -3' miRNA: 3'- -GUGagGGCUC-GCGGCGcGAGCGUGUA- -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 13213 | 0.69 | 0.232908 |
Target: 5'- cCACUCCCcguaguagaccauuGCGCCGCGaucCGCGCAa -3' miRNA: 3'- -GUGAGGGcu------------CGCGGCGCga-GCGUGUa -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 50724 | 0.69 | 0.232321 |
Target: 5'- cCGCUCCgGAuaGCCGCGCgcacgacggcCGCACGc -3' miRNA: 3'- -GUGAGGgCUcgCGGCGCGa---------GCGUGUa -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 36975 | 0.7 | 0.220834 |
Target: 5'- -uCUCCUGAgaucgGCGCgaugauggCGCGCUCGCGCGa -3' miRNA: 3'- guGAGGGCU-----CGCG--------GCGCGAGCGUGUa -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 6637 | 0.7 | 0.215274 |
Target: 5'- uCAUgCCCGAGCG-CGCGCUCGUcuGCu- -3' miRNA: 3'- -GUGaGGGCUCGCgGCGCGAGCG--UGua -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 40773 | 0.71 | 0.170461 |
Target: 5'- -cCUCCgGAGCGUagauCGCGCUgCGCACGc -3' miRNA: 3'- guGAGGgCUCGCG----GCGCGA-GCGUGUa -5' |
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22681 | 5' | -61.6 | NC_005091.1 | + | 6108 | 0.71 | 0.170461 |
Target: 5'- aACgCCCGAacguccGCGCCGCcCUCGUACAUa -3' miRNA: 3'- gUGaGGGCU------CGCGGCGcGAGCGUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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