Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
22691 | 3' | -61.4 | NC_005091.1 | + | 18151 | 0.77 | 0.076812 |
Target: 5'- gCGAGcUUcGCGGCCCGGCGaucgagcgUGAGCGUg -3' miRNA: 3'- -GCUC-AGcCGCCGGGCCGCa-------ACUCGCG- -5' |
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22691 | 3' | -61.4 | NC_005091.1 | + | 49821 | 0.78 | 0.063431 |
Target: 5'- cCGcGUucgUGGCGGCuuGcGCGUUGAGCGCg -3' miRNA: 3'- -GCuCA---GCCGCCGggC-CGCAACUCGCG- -5' |
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22691 | 3' | -61.4 | NC_005091.1 | + | 26654 | 1.11 | 0.000225 |
Target: 5'- uCGAGUCGGCGGCCCGGCGUUGAGCGCa -3' miRNA: 3'- -GCUCAGCCGCCGGGCCGCAACUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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