Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22692 | 3' | -62.4 | NC_005091.1 | + | 53994 | 0.66 | 0.348709 |
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Target: 5'- -cGGCUGGCG-CaauCCGCuGUCCGAc -3' miRNA: 3'- cuCCGACCGCaGcgcGGCGuCAGGCU- -5' |
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| 22692 | 3' | -62.4 | NC_005091.1 | + | 55810 | 0.66 | 0.348709 |
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Target: 5'- aGGGGCgcgGGcCGUCaGCGUCaCGGUCCa- -3' miRNA: 3'- -CUCCGa--CC-GCAG-CGCGGcGUCAGGcu -5' |
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| 22692 | 3' | -62.4 | NC_005091.1 | + | 29498 | 0.67 | 0.303248 |
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Target: 5'- gGAcGCUGGCG-CGCGCCGaucaCAGcUUCGAg -3' miRNA: 3'- -CUcCGACCGCaGCGCGGC----GUC-AGGCU- -5' |
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| 22692 | 3' | -62.4 | NC_005091.1 | + | 24325 | 0.68 | 0.282284 |
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Target: 5'- uAGGCUGGCcgucgaccGUCGUGCCGCcGaucuUCUGGu -3' miRNA: 3'- cUCCGACCG--------CAGCGCGGCGuC----AGGCU- -5' |
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| 22692 | 3' | -62.4 | NC_005091.1 | + | 50296 | 0.68 | 0.268957 |
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Target: 5'- -uGGCucuaucUGGCGUCGCGacaCGCGcaaggugcauccGUCCGAu -3' miRNA: 3'- cuCCG------ACCGCAGCGCg--GCGU------------CAGGCU- -5' |
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| 22692 | 3' | -62.4 | NC_005091.1 | + | 3085 | 0.68 | 0.262485 |
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Target: 5'- aGAGGCaacGCGUCGCcCUGCAauucGUCCGGu -3' miRNA: 3'- -CUCCGac-CGCAGCGcGGCGU----CAGGCU- -5' |
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| 22692 | 3' | -62.4 | NC_005091.1 | + | 12329 | 0.68 | 0.262485 |
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Target: 5'- --aGUUGGCGUCGUGuuGCAGagCUGGa -3' miRNA: 3'- cucCGACCGCAGCGCggCGUCa-GGCU- -5' |
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| 22692 | 3' | -62.4 | NC_005091.1 | + | 27247 | 1.08 | 0.000257 |
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Target: 5'- aGAGGCUGGCGUCGCGCCGCAGUCCGAg -3' miRNA: 3'- -CUCCGACCGCAGCGCGGCGUCAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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