Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22693 | 5' | -53.9 | NC_005091.1 | + | 28054 | 0.66 | 0.791395 |
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Target: 5'- gGCGAUCaGAUCaUGGGUGcguUCCGCUu -3' miRNA: 3'- gUGCUGGaCUAGcAUCCAU---AGGCGGc -5' |
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| 22693 | 5' | -53.9 | NC_005091.1 | + | 52871 | 0.67 | 0.780567 |
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Target: 5'- uGCGGCCUGAUCG-------CCGCCGc -3' miRNA: 3'- gUGCUGGACUAGCauccauaGGCGGC- -5' |
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| 22693 | 5' | -53.9 | NC_005091.1 | + | 21536 | 0.67 | 0.771569 |
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Target: 5'- -uCGGCUUGGUCcgGGGUGUagCCGCUGg -3' miRNA: 3'- guGCUGGACUAGcaUCCAUA--GGCGGC- -5' |
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| 22693 | 5' | -53.9 | NC_005091.1 | + | 5225 | 0.67 | 0.751172 |
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Target: 5'- gACGACCUGAUgGccGGUAUauugcgaGCCGu -3' miRNA: 3'- gUGCUGGACUAgCauCCAUAgg-----CGGC- -5' |
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| 22693 | 5' | -53.9 | NC_005091.1 | + | 574 | 0.68 | 0.719702 |
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Target: 5'- -cCGACCgcaaccGGUaCGUGGuGUAUCCGUCGc -3' miRNA: 3'- guGCUGGa-----CUA-GCAUC-CAUAGGCGGC- -5' |
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| 22693 | 5' | -53.9 | NC_005091.1 | + | 57967 | 0.68 | 0.719702 |
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Target: 5'- -cCGACCgcaaccGGUaCGUGGuGUAUCCGUCGc -3' miRNA: 3'- guGCUGGa-----CUA-GCAUC-CAUAGGCGGC- -5' |
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| 22693 | 5' | -53.9 | NC_005091.1 | + | 6004 | 0.69 | 0.665673 |
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Target: 5'- gCGCGcACCUGAcCGUuGGUcaauuucaagcGUCCGUCGg -3' miRNA: 3'- -GUGC-UGGACUaGCAuCCA-----------UAGGCGGC- -5' |
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| 22693 | 5' | -53.9 | NC_005091.1 | + | 30519 | 0.72 | 0.483028 |
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Target: 5'- aCGCGACCcGcguagCGUAGGUcAUCgGCCGg -3' miRNA: 3'- -GUGCUGGaCua---GCAUCCA-UAGgCGGC- -5' |
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| 22693 | 5' | -53.9 | NC_005091.1 | + | 28002 | 1.08 | 0.001847 |
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Target: 5'- aCACGACCUGAUCGUAGGUAUCCGCCGc -3' miRNA: 3'- -GUGCUGGACUAGCAUCCAUAGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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