Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 227 | 3' | -59.1 | AC_000008.1 | + | 11988 | 0.66 | 0.424657 |
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Target: 5'- cCGUCCcCGuCg--CCGC-CGCCUCCc -3' miRNA: 3'- -GCAGGcGCuGaaaGGCGcGCGGAGGu -5' |
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| 227 | 3' | -59.1 | AC_000008.1 | + | 19705 | 0.67 | 0.347831 |
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Target: 5'- gGUCgGCGGCgguggcgacgugCUaCGCGCCUCCGc -3' miRNA: 3'- gCAGgCGCUGaaa---------GGcGCGCGGAGGU- -5' |
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| 227 | 3' | -59.1 | AC_000008.1 | + | 15961 | 0.67 | 0.342856 |
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Target: 5'- aGUgCGCGGCgcgggcacuaCCGCGCGCC-CUg -3' miRNA: 3'- gCAgGCGCUGaaa-------GGCGCGCGGaGGu -5' |
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| 227 | 3' | -59.1 | AC_000008.1 | + | 20837 | 0.68 | 0.32032 |
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Target: 5'- ----gGCGGCUUUCCGCGgcuggGCCUUCAc -3' miRNA: 3'- gcaggCGCUGAAAGGCGCg----CGGAGGU- -5' |
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| 227 | 3' | -59.1 | AC_000008.1 | + | 19820 | 0.69 | 0.275824 |
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Target: 5'- gCGUggGCGugUagUUGCGCGCCUCCu -3' miRNA: 3'- -GCAggCGCugAaaGGCGCGCGGAGGu -5' |
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| 227 | 3' | -59.1 | AC_000008.1 | + | 25407 | 1.08 | 0.000323 |
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Target: 5'- gCGUCCGCGACUUUCCGCGCGCCUCCAc -3' miRNA: 3'- -GCAGGCGCUGAAAGGCGCGCGGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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