Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2270 | 3' | -62 | NC_001405.1 | + | 11786 | 0.66 | 0.25031 |
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Target: 5'- cCAGCGGCCcuCGGGCUCUacuaUGUUuagcagcgcaUCCCu -3' miRNA: 3'- -GUCGUCGGc-GCCCGGGA----ACGA----------AGGGu -5' |
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| 2270 | 3' | -62 | NC_001405.1 | + | 18794 | 0.66 | 0.243903 |
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Target: 5'- gAGCcGCCGUGcGCCC--GCUUUCCAa -3' miRNA: 3'- gUCGuCGGCGCcCGGGaaCGAAGGGU- -5' |
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| 2270 | 3' | -62 | NC_001405.1 | + | 16337 | 0.68 | 0.192219 |
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Target: 5'- gCAGCAGCCGCGG-CCauuagUGCUaugaCUCAg -3' miRNA: 3'- -GUCGUCGGCGCCcGGga---ACGAa---GGGU- -5' |
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| 2270 | 3' | -62 | NC_001405.1 | + | 16257 | 0.68 | 0.192219 |
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Target: 5'- gGGCGGCCauGCGGGCCgCUcgaagGCUggCCGc -3' miRNA: 3'- gUCGUCGG--CGCCCGG-GAa----CGAagGGU- -5' |
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| 2270 | 3' | -62 | NC_001405.1 | + | 16407 | 0.68 | 0.192219 |
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Target: 5'- uUAGCGGCCuGCGcGuGCCCgUGCgcaCCCGc -3' miRNA: 3'- -GUCGUCGG-CGC-C-CGGGaACGaa-GGGU- -5' |
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| 2270 | 3' | -62 | NC_001405.1 | + | 13946 | 0.69 | 0.158907 |
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Target: 5'- gGGCGGgCGCGGGCCgg-GCacaUCCCu -3' miRNA: 3'- gUCGUCgGCGCCCGGgaaCGa--AGGGu -5' |
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| 2270 | 3' | -62 | NC_001405.1 | + | 15877 | 0.71 | 0.101576 |
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Target: 5'- cCAGCaauaacacaGGCUG-GGGCCUgcGCUUCCCAa -3' miRNA: 3'- -GUCG---------UCGGCgCCCGGGaaCGAAGGGU- -5' |
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| 2270 | 3' | -62 | NC_001405.1 | + | 12433 | 0.72 | 0.090634 |
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Target: 5'- gCAGCAGCCGCaGGCCaaccgGCUcUCCgCAa -3' miRNA: 3'- -GUCGUCGGCGcCCGGgaa--CGA-AGG-GU- -5' |
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| 2270 | 3' | -62 | NC_001405.1 | + | 26184 | 1.07 | 0.000134 |
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Target: 5'- uCAGCAGCCGCGGGCCCUUGCUUCCCAg -3' miRNA: 3'- -GUCGUCGGCGCCCGGGAACGAAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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