Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22700 | 5' | -52.4 | NC_005091.1 | + | 17928 | 0.66 | 0.864684 |
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Target: 5'- cGGUCgugcgguaGACGG-CCGGCGCgac--GGGu -3' miRNA: 3'- -UCAGa-------CUGCCuGGUCGCGaaauuCCC- -5' |
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| 22700 | 5' | -52.4 | NC_005091.1 | + | 31077 | 0.66 | 0.864684 |
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Target: 5'- --aCUGACGGG-CGGCGCagcguGGGa -3' miRNA: 3'- ucaGACUGCCUgGUCGCGaaauuCCC- -5' |
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| 22700 | 5' | -52.4 | NC_005091.1 | + | 37139 | 0.66 | 0.864684 |
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Target: 5'- aAGUCUucGuCGGcCCGGCGCagUGcGGGa -3' miRNA: 3'- -UCAGA--CuGCCuGGUCGCGaaAUuCCC- -5' |
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| 22700 | 5' | -52.4 | NC_005091.1 | + | 40582 | 0.67 | 0.797648 |
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Target: 5'- cGGUCUGugGGGCUA-CGCggagaacauGGGu -3' miRNA: 3'- -UCAGACugCCUGGUcGCGaaauu----CCC- -5' |
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| 22700 | 5' | -52.4 | NC_005091.1 | + | 30128 | 0.72 | 0.521355 |
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Target: 5'- -cUCUGGCGGuAUCAGCGCUUUAugcugaccccgucacGGGu -3' miRNA: 3'- ucAGACUGCC-UGGUCGCGAAAUu--------------CCC- -5' |
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| 22700 | 5' | -52.4 | NC_005091.1 | + | 30080 | 1.1 | 0.001801 |
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Target: 5'- cAGUCUGACGGACCAGCGCUUUAAGGGc -3' miRNA: 3'- -UCAGACUGCCUGGUCGCGAAAUUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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