Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22701 | 5' | -48.3 | NC_005091.1 | + | 16343 | 0.66 | 0.985882 |
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Target: 5'- gUGCUcgGcGCGUUCgucgCGAGCGgcgcAUCGc -3' miRNA: 3'- gACGAa-C-CGCAAGaa--GCUUGCa---UAGC- -5' |
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| 22701 | 5' | -48.3 | NC_005091.1 | + | 47741 | 0.66 | 0.98391 |
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Target: 5'- uUGC-UGGCGUUCag-GAGCGUcgaggaaacGUCGa -3' miRNA: 3'- gACGaACCGCAAGaagCUUGCA---------UAGC- -5' |
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| 22701 | 5' | -48.3 | NC_005091.1 | + | 3342 | 0.66 | 0.981736 |
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Target: 5'- uUGCUUccGGCGUgucccUUUUCGAAggcaugccgcuCGUGUCGc -3' miRNA: 3'- gACGAA--CCGCA-----AGAAGCUU-----------GCAUAGC- -5' |
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| 22701 | 5' | -48.3 | NC_005091.1 | + | 1283 | 0.67 | 0.970802 |
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Target: 5'- -gGCa-GGUGUUCUUCGcGACGUG-CGg -3' miRNA: 3'- gaCGaaCCGCAAGAAGC-UUGCAUaGC- -5' |
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| 22701 | 5' | -48.3 | NC_005091.1 | + | 32317 | 0.67 | 0.970479 |
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Target: 5'- cCUGCUUGGCGcUCgcggUCGGccuugauagcagcACGgcguUCGg -3' miRNA: 3'- -GACGAACCGCaAGa---AGCU-------------UGCau--AGC- -5' |
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| 22701 | 5' | -48.3 | NC_005091.1 | + | 16313 | 0.68 | 0.95582 |
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Target: 5'- aCUGgUUGGCGUUgUUCGGuGCGUccggaAUCc -3' miRNA: 3'- -GACgAACCGCAAgAAGCU-UGCA-----UAGc -5' |
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| 22701 | 5' | -48.3 | NC_005091.1 | + | 2305 | 0.7 | 0.890743 |
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Target: 5'- -gGCUUGGCGUcuUCcacgUCGAGCGUc--- -3' miRNA: 3'- gaCGAACCGCA--AGa---AGCUUGCAuagc -5' |
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| 22701 | 5' | -48.3 | NC_005091.1 | + | 9027 | 0.7 | 0.890743 |
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Target: 5'- -cGUUU-GCGUUCUUCGAGCGUcuuuuUCa -3' miRNA: 3'- gaCGAAcCGCAAGAAGCUUGCAu----AGc -5' |
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| 22701 | 5' | -48.3 | NC_005091.1 | + | 32159 | 0.72 | 0.821906 |
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Target: 5'- -cGCcgUGGCGUUCUU-GcuCGUAUCGg -3' miRNA: 3'- gaCGa-ACCGCAAGAAgCuuGCAUAGC- -5' |
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| 22701 | 5' | -48.3 | NC_005091.1 | + | 31611 | 1.11 | 0.004473 |
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Target: 5'- gCUGCUUGGCGUUCUUCGAACGUAUCGg -3' miRNA: 3'- -GACGAACCGCAAGAAGCUUGCAUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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