Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22702 | 3' | -50.1 | NC_005091.1 | + | 37390 | 0.67 | 0.947422 |
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Target: 5'- uCGCGACaGCGCGGgaaGCgUUUUUGaAGGa -3' miRNA: 3'- cGCGCUGcUGUGCCa--UG-AAAAGC-UCC- -5' |
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| 22702 | 3' | -50.1 | NC_005091.1 | + | 31923 | 0.67 | 0.951969 |
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Target: 5'- uCGUGACGACGCGG-GCgaa-CGuGGc -3' miRNA: 3'- cGCGCUGCUGUGCCaUGaaaaGCuCC- -5' |
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| 22702 | 3' | -50.1 | NC_005091.1 | + | 26569 | 0.67 | 0.947422 |
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Target: 5'- uCGCGACGcGCugGGUGagucgUUCGAa- -3' miRNA: 3'- cGCGCUGC-UGugCCAUgaa--AAGCUcc -5' |
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| 22702 | 3' | -50.1 | NC_005091.1 | + | 1947 | 0.67 | 0.9426 |
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Target: 5'- aCGCGACGAUGCGGccGCcgugUCGAa- -3' miRNA: 3'- cGCGCUGCUGUGCCa-UGaaa-AGCUcc -5' |
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| 22702 | 3' | -50.1 | NC_005091.1 | + | 53909 | 0.66 | 0.960253 |
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Target: 5'- uGCGCGaACG--GCGGUACgcUUUCGAa- -3' miRNA: 3'- -CGCGC-UGCugUGCCAUGa-AAAGCUcc -5' |
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| 22702 | 3' | -50.1 | NC_005091.1 | + | 29065 | 0.66 | 0.97074 |
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Target: 5'- gGCGUGACGccuuccaggauGgGCGGUgaGCUUUUUcAGGa -3' miRNA: 3'- -CGCGCUGC-----------UgUGCCA--UGAAAAGcUCC- -5' |
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| 22702 | 3' | -50.1 | NC_005091.1 | + | 48767 | 0.66 | 0.963637 |
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Target: 5'- aCGCGGCaggccguGACACGG-GCaa-UCGGGGa -3' miRNA: 3'- cGCGCUG-------CUGUGCCaUGaaaAGCUCC- -5' |
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| 22702 | 3' | -50.1 | NC_005091.1 | + | 23493 | 0.66 | 0.964001 |
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Target: 5'- cGCGCGGCGAUggcgcugcuCGGU-CUgUUCGGcGGc -3' miRNA: 3'- -CGCGCUGCUGu--------GCCAuGAaAAGCU-CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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