Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22704 | 3' | -56.2 | NC_005091.1 | + | 35140 | 0.7 | 0.46349 |
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Target: 5'- cUUGugGUacGCGUCGCGGCgccGCCgCGAg -3' miRNA: 3'- uAGCugCA--CGUAGCGCUGa--UGGgGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 20949 | 0.7 | 0.424856 |
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Target: 5'- -aCGACGUGCugaAUC-CGACUGCCgUCGAa -3' miRNA: 3'- uaGCUGCACG---UAGcGCUGAUGG-GGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 16205 | 0.7 | 0.462502 |
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Target: 5'- gAUCGG-GUGCAUCucauGCGGCUGgccucguCCCCGAu -3' miRNA: 3'- -UAGCUgCACGUAG----CGCUGAU-------GGGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 25113 | 0.71 | 0.377628 |
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Target: 5'- cAUCGACGcgccggcUGCAUCgacgcccggugcaGCGGCcGCCCCGGa -3' miRNA: 3'- -UAGCUGC-------ACGUAG-------------CGCUGaUGGGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 50808 | 0.72 | 0.321418 |
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Target: 5'- -cCGuCGUGCG-CGCGGCUAUCCgGAg -3' miRNA: 3'- uaGCuGCACGUaGCGCUGAUGGGgCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 26091 | 0.74 | 0.244202 |
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Target: 5'- cGUCGGCGUGCAgggCGCGAUUaACCuuGc -3' miRNA: 3'- -UAGCUGCACGUa--GCGCUGA-UGGggCu -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 23466 | 0.75 | 0.208968 |
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Target: 5'- uUCGGCG-GCGcugCGuCGGCUGCCCCGGc -3' miRNA: 3'- uAGCUGCaCGUa--GC-GCUGAUGGGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 32735 | 1.06 | 0.001357 |
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Target: 5'- uAUCGACGUGCAUCGCGACUACCCCGAu -3' miRNA: 3'- -UAGCUGCACGUAGCGCUGAUGGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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