Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22704 | 3' | -56.2 | NC_005091.1 | + | 14051 | 0.69 | 0.473434 |
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Target: 5'- -aCGACG-GUAUCGCGuaucACUGcgacCCCCGAg -3' miRNA: 3'- uaGCUGCaCGUAGCGC----UGAU----GGGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 9085 | 0.66 | 0.653859 |
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Target: 5'- -cCGugGgucgGCGUCGUG-CUgaagcgcaACCCCGAu -3' miRNA: 3'- uaGCugCa---CGUAGCGCuGA--------UGGGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 6644 | 0.66 | 0.707754 |
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Target: 5'- uUCGAUGUGgGccuucgauuUCG-GGCUGCCCCa- -3' miRNA: 3'- uAGCUGCACgU---------AGCgCUGAUGGGGcu -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 2589 | 0.66 | 0.664718 |
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Target: 5'- cUCGAUG-GCAUCGC-ACUgguugGCUCCGGu -3' miRNA: 3'- uAGCUGCaCGUAGCGcUGA-----UGGGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 2384 | 0.67 | 0.621201 |
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Target: 5'- uUCGGCGUcgucgcgauGCGUCGUGGCUucuuGCCaCUGGa -3' miRNA: 3'- uAGCUGCA---------CGUAGCGCUGA----UGG-GGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 2304 | 0.69 | 0.503884 |
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Target: 5'- -aCGAC--GCAUCGCGACgACgCCGAa -3' miRNA: 3'- uaGCUGcaCGUAGCGCUGaUGgGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 655 | 0.66 | 0.696005 |
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Target: 5'- aAUCGACcggGCAUCGCGgaaaucuacgcggACUGCgCCGc -3' miRNA: 3'- -UAGCUGca-CGUAGCGC-------------UGAUGgGGCu -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 77 | 0.67 | 0.621201 |
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Target: 5'- --aGGCGUGCGcCaCGGCUGCCUCGu -3' miRNA: 3'- uagCUGCACGUaGcGCUGAUGGGGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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