Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22704 | 3' | -56.2 | NC_005091.1 | + | 52125 | 0.67 | 0.603803 |
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Target: 5'- -gCGGCGcGUAucUCGCGAUUgaggaagucacgcucGCCCCGAa -3' miRNA: 3'- uaGCUGCaCGU--AGCGCUGA---------------UGGGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 50665 | 0.67 | 0.621201 |
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Target: 5'- -gCGACGcUGUugCGCGACUACCgcaCCGGc -3' miRNA: 3'- uaGCUGC-ACGuaGCGCUGAUGG---GGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 2384 | 0.67 | 0.621201 |
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Target: 5'- uUCGGCGUcgucgcgauGCGUCGUGGCUucuuGCCaCUGGa -3' miRNA: 3'- uAGCUGCA---------CGUAGCGCUGA----UGG-GGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 49489 | 0.67 | 0.63209 |
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Target: 5'- gAUCGAaauugcuaagaGUGCcgCGCGugUucagcgcgaGCCCCGGc -3' miRNA: 3'- -UAGCUg----------CACGuaGCGCugA---------UGGGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 44574 | 0.66 | 0.675548 |
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Target: 5'- cUCGACGUGCA--GCGAagaAgCCCGGu -3' miRNA: 3'- uAGCUGCACGUagCGCUga-UgGGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 55909 | 0.66 | 0.686338 |
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Target: 5'- -cCGGC-UGCgccgcucuucGUCGCGuACUugCCCGAg -3' miRNA: 3'- uaGCUGcACG----------UAGCGC-UGAugGGGCU- -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 6644 | 0.66 | 0.707754 |
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Target: 5'- uUCGAUGUGgGccuucgauuUCG-GGCUGCCCCa- -3' miRNA: 3'- uAGCUGCACgU---------AGCgCUGAUGGGGcu -5' |
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| 22704 | 3' | -56.2 | NC_005091.1 | + | 23466 | 0.75 | 0.208968 |
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Target: 5'- uUCGGCG-GCGcugCGuCGGCUGCCCCGGc -3' miRNA: 3'- uAGCUGCaCGUa--GC-GCUGAUGGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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