Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22704 | 5' | -58.7 | NC_005091.1 | + | 14226 | 0.66 | 0.598897 |
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Target: 5'- aUCgUGAcGGGCacgGUCGAG-CCGCCUUgCGu -3' miRNA: 3'- -AGgGCU-CCCG---CAGCUCuGGUGGAA-GC- -5' |
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| 22704 | 5' | -58.7 | NC_005091.1 | + | 29785 | 0.66 | 0.577847 |
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Target: 5'- cUCCGGGGaGUuUCGggacuacguGGACCGCCUUCc -3' miRNA: 3'- aGGGCUCC-CGcAGC---------UCUGGUGGAAGc -5' |
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| 22704 | 5' | -58.7 | NC_005091.1 | + | 49558 | 0.67 | 0.505836 |
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Target: 5'- -gUCGGGGGCGUCuucGuACCGCCUgcgCGg -3' miRNA: 3'- agGGCUCCCGCAGcu-C-UGGUGGAa--GC- -5' |
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| 22704 | 5' | -58.7 | NC_005091.1 | + | 19998 | 0.67 | 0.505836 |
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Target: 5'- -gCCGuGGGCGgcgauugguUCGAGugCGCCgaggCGg -3' miRNA: 3'- agGGCuCCCGC---------AGCUCugGUGGaa--GC- -5' |
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| 22704 | 5' | -58.7 | NC_005091.1 | + | 25200 | 0.67 | 0.492864 |
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Target: 5'- -aCCGGGGucgaugcagccggcGCGUCGAuggcGACCGCCUcCGu -3' miRNA: 3'- agGGCUCC--------------CGCAGCU----CUGGUGGAaGC- -5' |
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| 22704 | 5' | -58.7 | NC_005091.1 | + | 17366 | 0.68 | 0.466429 |
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Target: 5'- aCCUGAucuucacauGGGCGcaUCGAGACCGCa-UCGc -3' miRNA: 3'- aGGGCU---------CCCGC--AGCUCUGGUGgaAGC- -5' |
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| 22704 | 5' | -58.7 | NC_005091.1 | + | 32200 | 0.69 | 0.41048 |
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Target: 5'- cUUCCGGuGcGGCGgcagCGAGcAUCACCUUCGc -3' miRNA: 3'- -AGGGCU-C-CCGCa---GCUC-UGGUGGAAGC- -5' |
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| 22704 | 5' | -58.7 | NC_005091.1 | + | 7079 | 0.71 | 0.319496 |
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Target: 5'- cUCCUGaAGGGCaUCGAGACgGCCgcCGc -3' miRNA: 3'- -AGGGC-UCCCGcAGCUCUGgUGGaaGC- -5' |
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| 22704 | 5' | -58.7 | NC_005091.1 | + | 32774 | 1.08 | 0.00068 |
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Target: 5'- cUCCCGAGGGCGUCGAGACCACCUUCGu -3' miRNA: 3'- -AGGGCUCCCGCAGCUCUGGUGGAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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