Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22706 | 3' | -59.3 | NC_005091.1 | + | 31203 | 0.66 | 0.465343 |
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Target: 5'- aUgCCGCcgaugaUCGGCuGcUCGCCGGCACg- -3' miRNA: 3'- cAgGGUG------AGCCGuC-AGCGGUCGUGaa -5' |
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| 22706 | 3' | -59.3 | NC_005091.1 | + | 25096 | 0.66 | 0.455495 |
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Target: 5'- --gCCACggaGGCGGUCGCCAucgacGCGCc- -3' miRNA: 3'- cagGGUGag-CCGUCAGCGGU-----CGUGaa -5' |
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| 22706 | 3' | -59.3 | NC_005091.1 | + | 40374 | 0.67 | 0.426648 |
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Target: 5'- aUCCUGC-CGaGCGGUCGCCGgauGUACUa -3' miRNA: 3'- cAGGGUGaGC-CGUCAGCGGU---CGUGAa -5' |
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| 22706 | 3' | -59.3 | NC_005091.1 | + | 21856 | 0.67 | 0.426648 |
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Target: 5'- cGUCa-GCUCGGCAGUUGUCAcguuccagaGCACg- -3' miRNA: 3'- -CAGggUGAGCCGUCAGCGGU---------CGUGaa -5' |
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| 22706 | 3' | -59.3 | NC_005091.1 | + | 888 | 0.68 | 0.37236 |
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Target: 5'- -aCCCACgCGGCAG-CGC-AGCGCa- -3' miRNA: 3'- caGGGUGaGCCGUCaGCGgUCGUGaa -5' |
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| 22706 | 3' | -59.3 | NC_005091.1 | + | 28522 | 0.68 | 0.355338 |
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Target: 5'- --gUCGCUCgaacuGGCcGUCGCCAGCACg- -3' miRNA: 3'- cagGGUGAG-----CCGuCAGCGGUCGUGaa -5' |
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| 22706 | 3' | -59.3 | NC_005091.1 | + | 30687 | 0.71 | 0.239276 |
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Target: 5'- uUCCUGCUCGGUGuugucGUCGCCGGUAUa- -3' miRNA: 3'- cAGGGUGAGCCGU-----CAGCGGUCGUGaa -5' |
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| 22706 | 3' | -59.3 | NC_005091.1 | + | 38238 | 0.73 | 0.160943 |
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Target: 5'- cGUCUCGCcCGGCAGg-GCCAGCGCc- -3' miRNA: 3'- -CAGGGUGaGCCGUCagCGGUCGUGaa -5' |
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| 22706 | 3' | -59.3 | NC_005091.1 | + | 34475 | 1.04 | 0.000782 |
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Target: 5'- gGUCCCACUCGGCAGUCGCCAGCACUUc -3' miRNA: 3'- -CAGGGUGAGCCGUCAGCGGUCGUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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