Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2271 | 3' | -55.4 | NC_001405.1 | + | 12158 | 0.66 | 0.586856 |
Target: 5'- -gGAGGCagCUGGGGCCGGaccugggcuGGcGGUGg -3' miRNA: 3'- ugCUUCGaaGGCUCCGGCU---------UCuCCAC- -5' |
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2271 | 3' | -55.4 | NC_001405.1 | + | 15184 | 0.69 | 0.416645 |
Target: 5'- -aGAAGCgcgCUGAGGCCGAGGcAGcGg- -3' miRNA: 3'- ugCUUCGaa-GGCUCCGGCUUC-UC-Cac -5' |
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2271 | 3' | -55.4 | NC_001405.1 | + | 2331 | 0.76 | 0.140739 |
Target: 5'- -gGggGCUUCUGAGGCUacaGAGGAGGc- -3' miRNA: 3'- ugCuuCGAAGGCUCCGG---CUUCUCCac -5' |
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2271 | 3' | -55.4 | NC_001405.1 | + | 26339 | 1.08 | 0.000677 |
Target: 5'- gACGAAGCUUCCGAGGCCGAAGAGGUGu -3' miRNA: 3'- -UGCUUCGAAGGCUCCGGCUUCUCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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