Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22710 | 5' | -60.7 | NC_005091.1 | + | 32184 | 0.66 | 0.458031 |
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Target: 5'- cGGAguCGGcGC-GGACc-CGGCCGGCGg -3' miRNA: 3'- cCCU--GCC-CGaCCUGaaGCCGGUCGCa -5' |
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| 22710 | 5' | -60.7 | NC_005091.1 | + | 16134 | 0.66 | 0.44856 |
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Target: 5'- cGGGAagauggaacgUGGGUUGuGCcgcCGGCCGGCGg -3' miRNA: 3'- -CCCU----------GCCCGACcUGaa-GCCGGUCGCa -5' |
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| 22710 | 5' | -60.7 | NC_005091.1 | + | 23716 | 0.66 | 0.429948 |
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Target: 5'- cGGaGAUGucGCcGGGCUUCGGCCAuCGUa -3' miRNA: 3'- -CC-CUGCc-CGaCCUGAAGCCGGUcGCA- -5' |
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| 22710 | 5' | -60.7 | NC_005091.1 | + | 26219 | 0.66 | 0.429948 |
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Target: 5'- -cGACGcGGC-GGcaGCUUCGGCCugAGCGg -3' miRNA: 3'- ccCUGC-CCGaCC--UGAAGCCGG--UCGCa -5' |
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| 22710 | 5' | -60.7 | NC_005091.1 | + | 28122 | 0.68 | 0.368568 |
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Target: 5'- aGuuCGuGaGCgGGAUUUCGGCCGGCGUg -3' miRNA: 3'- cCcuGC-C-CGaCCUGAAGCCGGUCGCA- -5' |
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| 22710 | 5' | -60.7 | NC_005091.1 | + | 34070 | 0.68 | 0.352169 |
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Target: 5'- cGGGCGGcaGCUcGGACUcgauagCGGCC-GCGUa -3' miRNA: 3'- cCCUGCC--CGA-CCUGAa-----GCCGGuCGCA- -5' |
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| 22710 | 5' | -60.7 | NC_005091.1 | + | 16991 | 0.7 | 0.271399 |
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Target: 5'- uGGugGGGCUGGGCggaCGG--AGCGUc -3' miRNA: 3'- cCCugCCCGACCUGaa-GCCggUCGCA- -5' |
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| 22710 | 5' | -60.7 | NC_005091.1 | + | 53513 | 0.7 | 0.264845 |
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Target: 5'- cGGGAUGGuCUaaGGGCgaaugcaCGGCCAGCGa -3' miRNA: 3'- -CCCUGCCcGA--CCUGaa-----GCCGGUCGCa -5' |
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| 22710 | 5' | -60.7 | NC_005091.1 | + | 56096 | 0.74 | 0.139438 |
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Target: 5'- --cGCGGGCgGGAggUCGGCCGGCGc -3' miRNA: 3'- cccUGCCCGaCCUgaAGCCGGUCGCa -5' |
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| 22710 | 5' | -60.7 | NC_005091.1 | + | 36652 | 1.08 | 0.000373 |
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Target: 5'- cGGGACGGGCUGGACUUCGGCCAGCGUc -3' miRNA: 3'- -CCCUGCCCGACCUGAAGCCGGUCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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