Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22711 | 5' | -59.5 | NC_005091.1 | + | 16998 | 0.66 | 0.5298 |
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Target: 5'- cGGCGUcuggUGgGGCUGggCGGACGGa- -3' miRNA: 3'- uCCGUAu---GCgCCGGCuaGCCUGCCgg -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 22841 | 0.66 | 0.499526 |
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Target: 5'- gAGcGCAcGCGCcugaacGCCGcgCuGACGGCCg -3' miRNA: 3'- -UC-CGUaUGCGc-----CGGCuaGcCUGCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 43120 | 0.66 | 0.526741 |
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Target: 5'- gGGGCAa--GUGGCCGAUgacugcuuugaCGaccucacugcggacGACGGCCu -3' miRNA: 3'- -UCCGUaugCGCCGGCUA-----------GC--------------CUGCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 24880 | 0.66 | 0.519629 |
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Target: 5'- cGGCcUGCGCaGCaCGG-CGGAauuccuCGGCCu -3' miRNA: 3'- uCCGuAUGCGcCG-GCUaGCCU------GCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 50741 | 0.66 | 0.519629 |
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Target: 5'- cGGCGUcgaauaucUGCGGCaCGAgCGGAacgacgaacCGGCCg -3' miRNA: 3'- uCCGUAu-------GCGCCG-GCUaGCCU---------GCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 34047 | 0.66 | 0.513564 |
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Target: 5'- aAGGCGUACGuCGGCggugcgaacaacaucCaGAUCG-ACGGCg -3' miRNA: 3'- -UCCGUAUGC-GCCG---------------G-CUAGCcUGCCGg -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 14188 | 0.66 | 0.509536 |
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Target: 5'- cGGCuacUAC-CGGCCGGaaccgcuucgUGGACGGCa -3' miRNA: 3'- uCCGu--AUGcGCCGGCUa---------GCCUGCCGg -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 51151 | 0.66 | 0.550352 |
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Target: 5'- -aGCGacGCGCGG-CGAUU-GACGGCCg -3' miRNA: 3'- ucCGUa-UGCGCCgGCUAGcCUGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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