Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22711 | 5' | -59.5 | NC_005091.1 | + | 7202 | 0.68 | 0.422074 |
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Target: 5'- cGGCAgauggcgGCGCGuuaccuucgucuuGCCGGcagCGG-CGGCCg -3' miRNA: 3'- uCCGUa------UGCGC-------------CGGCUa--GCCuGCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 6594 | 0.74 | 0.172759 |
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Target: 5'- cAGGCAcgAUGUGGaUCGAgUCGGGCGGCa -3' miRNA: 3'- -UCCGUa-UGCGCC-GGCU-AGCCUGCCGg -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 6445 | 0.66 | 0.550352 |
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Target: 5'- aGGGCAgcgugcgcgcUGCGCugaaggaagcaGGCgCGA-CGGAUGGCg -3' miRNA: 3'- -UCCGU----------AUGCG-----------CCG-GCUaGCCUGCCGg -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 6026 | 0.66 | 0.5298 |
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Target: 5'- aGGGCG-GCGCGGaCGuUCGGGCGuuCg -3' miRNA: 3'- -UCCGUaUGCGCCgGCuAGCCUGCcgG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 5322 | 0.71 | 0.279364 |
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Target: 5'- cGGCcg-UGCGGCCagugauguGAUCcgGGGCGGCCg -3' miRNA: 3'- uCCGuauGCGCCGG--------CUAG--CCUGCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 3329 | 0.69 | 0.345869 |
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Target: 5'- --uCAUGCGCGGguugaagucaUCGAUCGaGCGGCCg -3' miRNA: 3'- uccGUAUGCGCC----------GGCUAGCcUGCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 2134 | 0.66 | 0.499526 |
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Target: 5'- cGGUAaGCGCgucGGCCGAagCGGAacUGGCg -3' miRNA: 3'- uCCGUaUGCG---CCGGCUa-GCCU--GCCGg -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 827 | 0.72 | 0.240944 |
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Target: 5'- uGGGUGUACgGCGG-CGAUCcuGCGGCCa -3' miRNA: 3'- -UCCGUAUG-CGCCgGCUAGccUGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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