Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22711 | 5' | -59.5 | NC_005091.1 | + | 2134 | 0.66 | 0.499526 |
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Target: 5'- cGGUAaGCGCgucGGCCGAagCGGAacUGGCg -3' miRNA: 3'- uCCGUaUGCG---CCGGCUa-GCCU--GCCGg -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 8505 | 0.67 | 0.479776 |
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Target: 5'- uGGGCGcACGCGuuCGAgcaucCGGgcuGCGGCCc -3' miRNA: 3'- -UCCGUaUGCGCcgGCUa----GCC---UGCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 21622 | 0.67 | 0.450891 |
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Target: 5'- -cGCAa--GCGGaUCGAccggCGGGCGGCCg -3' miRNA: 3'- ucCGUaugCGCC-GGCUa---GCCUGCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 51648 | 0.67 | 0.441476 |
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Target: 5'- cGGCuuuccaguuCGCGGCCauUCGGGCGaGCg -3' miRNA: 3'- uCCGuau------GCGCCGGcuAGCCUGC-CGg -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 46199 | 0.67 | 0.438673 |
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Target: 5'- cGGCcacgaGCGCGGCCucgaacucuggcacGAguggUCGGAgaCGGCCg -3' miRNA: 3'- uCCGua---UGCGCCGG--------------CU----AGCCU--GCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 16247 | 0.68 | 0.422986 |
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Target: 5'- cGGCAUGCGaUGcGCCGcUCGcGACGaacgcGCCg -3' miRNA: 3'- uCCGUAUGC-GC-CGGCuAGC-CUGC-----CGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 11858 | 0.68 | 0.413917 |
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Target: 5'- cGGCcgAauUGCGGCaUGAaCGGAUGGUCg -3' miRNA: 3'- uCCGuaU--GCGCCG-GCUaGCCUGCCGG- -5' |
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| 22711 | 5' | -59.5 | NC_005091.1 | + | 36775 | 1.12 | 0.000301 |
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Target: 5'- cAGGCAUACGCGGCCGAUCGGACGGCCa -3' miRNA: 3'- -UCCGUAUGCGCCGGCUAGCCUGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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