Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22712 | 5' | -53 | NC_005091.1 | + | 9298 | 0.66 | 0.877815 |
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Target: 5'- cCGACCggcUGGCCGUGcgaaugguuCCCGcCGGGa -3' miRNA: 3'- -GCUGGa--ACUGGCACau-------GGGCuGCUUc -5' |
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| 22712 | 5' | -53 | NC_005091.1 | + | 19184 | 0.66 | 0.853702 |
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Target: 5'- gCGGCgCUUuGCCGUGgcCCCGuCGAu- -3' miRNA: 3'- -GCUG-GAAcUGGCACauGGGCuGCUuc -5' |
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| 22712 | 5' | -53 | NC_005091.1 | + | 10058 | 0.67 | 0.809015 |
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Target: 5'- gGugCUUGGCUG---ACCgGACGAGGa -3' miRNA: 3'- gCugGAACUGGCacaUGGgCUGCUUC- -5' |
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| 22712 | 5' | -53 | NC_005091.1 | + | 38459 | 0.68 | 0.789779 |
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Target: 5'- uCGAUCUugUGAUCGUGUACCCaauGCGu-- -3' miRNA: 3'- -GCUGGA--ACUGGCACAUGGGc--UGCuuc -5' |
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| 22712 | 5' | -53 | NC_005091.1 | + | 55707 | 0.68 | 0.777918 |
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Target: 5'- aCGACCUgagcagcggaugGACCGUG-ACgCUGACGGc- -3' miRNA: 3'- -GCUGGAa-----------CUGGCACaUG-GGCUGCUuc -5' |
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| 22712 | 5' | -53 | NC_005091.1 | + | 6233 | 0.72 | 0.543806 |
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Target: 5'- uCGGCCUcGAUC-UGUACCCGAagcaGAAGu -3' miRNA: 3'- -GCUGGAaCUGGcACAUGGGCUg---CUUC- -5' |
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| 22712 | 5' | -53 | NC_005091.1 | + | 47936 | 0.73 | 0.491353 |
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Target: 5'- gGACCUUGGCCGUuuucaGCCCuuCGggGg -3' miRNA: 3'- gCUGGAACUGGCAca---UGGGcuGCuuC- -5' |
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| 22712 | 5' | -53 | NC_005091.1 | + | 37003 | 1.09 | 0.002392 |
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Target: 5'- uCGACCUUGACCGUGUACCCGACGAAGu -3' miRNA: 3'- -GCUGGAACUGGCACAUGGGCUGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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