Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22718 | 5' | -56.5 | NC_005091.1 | + | 23419 | 0.67 | 0.626961 |
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Target: 5'- cGgGACGuGGGGCCuuGG-CGCUUccgUGCCu -3' miRNA: 3'- -CgCUGU-CCUCGGuuCCaGCGGA---ACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 16873 | 0.66 | 0.67036 |
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Target: 5'- aGCuGCAGcGAGCCGuucguGGUCGUCccccaGCCc -3' miRNA: 3'- -CGcUGUC-CUCGGUu----CCAGCGGaa---CGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 28981 | 0.66 | 0.67036 |
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Target: 5'- aCGGCAGGGG-CAGGcGUCuCCgcGCCg -3' miRNA: 3'- cGCUGUCCUCgGUUC-CAGcGGaaCGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 10907 | 0.66 | 0.681147 |
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Target: 5'- cGCGuACGGGucgucgcuGCCGAGuGcaaugcgCGCCUUGgCCg -3' miRNA: 3'- -CGC-UGUCCu-------CGGUUC-Ca------GCGGAAC-GG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 50374 | 0.66 | 0.681147 |
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Target: 5'- cGCG-CGGGccGCCAAGcGUCGCgCgaUGCUg -3' miRNA: 3'- -CGCuGUCCu-CGGUUC-CAGCG-Ga-ACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 55477 | 0.66 | 0.691889 |
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Target: 5'- aGCGugGcc-GCCAugacuuccuuGGUCGCCUcguUGCCa -3' miRNA: 3'- -CGCugUccuCGGUu---------CCAGCGGA---ACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 26408 | 0.66 | 0.691889 |
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Target: 5'- uGCGGCAGccgcAGCCugcGUUGCCUgcGCCu -3' miRNA: 3'- -CGCUGUCc---UCGGuucCAGCGGAa-CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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