Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22718 | 5' | -56.5 | NC_005091.1 | + | 43080 | 1.12 | 0.000469 |
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Target: 5'- uGCGACAGGAGCCAAGGUCGCCUUGCCc -3' miRNA: 3'- -CGCUGUCCUCGGUUCCAGCGGAACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 44613 | 0.7 | 0.430237 |
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Target: 5'- cCGGCuugaucGAGgCGAGGUCuucgGCCUUGCCg -3' miRNA: 3'- cGCUGuc----CUCgGUUCCAG----CGGAACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 46540 | 0.72 | 0.317517 |
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Target: 5'- cGCGACGGcuuGUCGAGcGUCGCCUUcuugaucGCCu -3' miRNA: 3'- -CGCUGUCcu-CGGUUC-CAGCGGAA-------CGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 50374 | 0.66 | 0.681147 |
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Target: 5'- cGCG-CGGGccGCCAAGcGUCGCgCgaUGCUg -3' miRNA: 3'- -CGCuGUCCu-CGGUUC-CAGCG-Ga-ACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 54861 | 0.67 | 0.600916 |
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Target: 5'- uGCGgaaAGGGGCUAGGcaagguuuaacuuuGUCGCCUagaaucucagucaUGCCg -3' miRNA: 3'- -CGCug-UCCUCGGUUC--------------CAGCGGA-------------ACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 55477 | 0.66 | 0.691889 |
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Target: 5'- aGCGugGcc-GCCAugacuuccuuGGUCGCCUcguUGCCa -3' miRNA: 3'- -CGCugUccuCGGUu---------CCAGCGGA---ACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 56492 | 0.75 | 0.201589 |
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Target: 5'- ----gGGGAGCCGAGcGUUgaGCCUUGCCa -3' miRNA: 3'- cgcugUCCUCGGUUC-CAG--CGGAACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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