Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22718 | 5' | -56.5 | NC_005091.1 | + | 11094 | 0.71 | 0.393373 |
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Target: 5'- -gGACGGGAGUCGGGaUCGCUuUUGCUu -3' miRNA: 3'- cgCUGUCCUCGGUUCcAGCGG-AACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 11010 | 0.77 | 0.158503 |
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Target: 5'- cCGGCuuc-GCCGGGGUCGCUUUGCCg -3' miRNA: 3'- cGCUGuccuCGGUUCCAGCGGAACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 11001 | 0.69 | 0.508551 |
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Target: 5'- cCGACGGGuuuccGCCGGGGcuucuuuUUGCCUacagGCCg -3' miRNA: 3'- cGCUGUCCu----CGGUUCC-------AGCGGAa---CGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 10907 | 0.66 | 0.681147 |
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Target: 5'- cGCGuACGGGucgucgcuGCCGAGuGcaaugcgCGCCUUGgCCg -3' miRNA: 3'- -CGC-UGUCCu-------CGGUUC-Ca------GCGGAAC-GG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 8014 | 0.69 | 0.479036 |
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Target: 5'- cGCGAgGGGA-UCGAGGUCGgCCU-GCa -3' miRNA: 3'- -CGCUgUCCUcGGUUCCAGC-GGAaCGg -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 2313 | 0.77 | 0.162845 |
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Target: 5'- cGCGACGac-GCCgAAGGUCGCCUgaaUGCCa -3' miRNA: 3'- -CGCUGUccuCGG-UUCCAGCGGA---ACGG- -5' |
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| 22718 | 5' | -56.5 | NC_005091.1 | + | 1960 | 0.7 | 0.402396 |
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Target: 5'- uGCGGCAaaguucgucuuGGAacggGCCGAGGUCGcCCUUGa- -3' miRNA: 3'- -CGCUGU-----------CCU----CGGUUCCAGC-GGAACgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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