Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22719 | 3' | -57.4 | NC_005091.1 | + | 23474 | 0.66 | 0.613488 |
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Target: 5'- -uCGGUCUg---UUCGGCGGCgCUGCg -3' miRNA: 3'- uuGCCAGAagggAAGCCGCUG-GACGa -5' |
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| 22719 | 3' | -57.4 | NC_005091.1 | + | 48232 | 0.66 | 0.591717 |
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Target: 5'- -uCGGUUcggCCCUUCGG--ACCUGCa -3' miRNA: 3'- uuGCCAGaa-GGGAAGCCgcUGGACGa -5' |
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| 22719 | 3' | -57.4 | NC_005091.1 | + | 9225 | 0.67 | 0.538015 |
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Target: 5'- cGCGGUgUUUCCggCGGUGcggcCCUGCg -3' miRNA: 3'- uUGCCAgAAGGGaaGCCGCu---GGACGa -5' |
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| 22719 | 3' | -57.4 | NC_005091.1 | + | 34387 | 0.67 | 0.527461 |
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Target: 5'- --aGGUCgcgcaCCC--CGGCGACCUGUa -3' miRNA: 3'- uugCCAGaa---GGGaaGCCGCUGGACGa -5' |
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| 22719 | 3' | -57.4 | NC_005091.1 | + | 31874 | 0.68 | 0.436658 |
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Target: 5'- -cCGuGUCgucgcgCUCgUCGGCGGCCUGCUc -3' miRNA: 3'- uuGC-CAGaa----GGGaAGCCGCUGGACGA- -5' |
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| 22719 | 3' | -57.4 | NC_005091.1 | + | 26644 | 0.69 | 0.427122 |
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Target: 5'- gGGCGGUCga-UCgagUCGGCGGCCcgGCg -3' miRNA: 3'- -UUGCCAGaagGGa--AGCCGCUGGa-CGa -5' |
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| 22719 | 3' | -57.4 | NC_005091.1 | + | 44114 | 0.69 | 0.399272 |
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Target: 5'- gAAUGGgcgcagucgCUUCCC---GGCGACCUGCa -3' miRNA: 3'- -UUGCCa--------GAAGGGaagCCGCUGGACGa -5' |
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| 22719 | 3' | -57.4 | NC_005091.1 | + | 24895 | 0.79 | 0.097483 |
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Target: 5'- gAACGG-CUUCC--UCGGCGGCCUGCg -3' miRNA: 3'- -UUGCCaGAAGGgaAGCCGCUGGACGa -5' |
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| 22719 | 3' | -57.4 | NC_005091.1 | + | 44107 | 1.05 | 0.001229 |
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Target: 5'- aAACGGUCUUCCCUUCGGCGACCUGCUc -3' miRNA: 3'- -UUGCCAGAAGGGAAGCCGCUGGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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