Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22720 | 3' | -55.5 | NC_005091.1 | + | 48970 | 0.66 | 0.694216 |
Target: 5'- uUG-GGGCU---UGGCGGGCGCGCUa- -3' miRNA: 3'- -ACaCUCGGuguACCGUCUGCGUGAgc -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 17017 | 0.66 | 0.705055 |
Target: 5'- cGUGAGCCA-GUGGguGAaggugaaguCGUACUUc -3' miRNA: 3'- aCACUCGGUgUACCguCU---------GCGUGAGc -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 49506 | 0.66 | 0.715824 |
Target: 5'- --cGAGCgGCA-GGCAGaACGCGC-Ca -3' miRNA: 3'- acaCUCGgUGUaCCGUC-UGCGUGaGc -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 4838 | 0.66 | 0.672366 |
Target: 5'- cGUGcAGUgACGUGGC-GAUcCGCUCGg -3' miRNA: 3'- aCAC-UCGgUGUACCGuCUGcGUGAGC- -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 46159 | 0.67 | 0.617259 |
Target: 5'- cGUGGGUCaACGUcGGCAuGGCGCugUUu -3' miRNA: 3'- aCACUCGG-UGUA-CCGU-CUGCGugAGc -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 51580 | 0.67 | 0.628294 |
Target: 5'- cGUGcGUCGCGUGaCGGGCGCGCa-- -3' miRNA: 3'- aCACuCGGUGUACcGUCUGCGUGagc -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 53274 | 0.67 | 0.606238 |
Target: 5'- ----cGCCGCAcuuugGGCGGAUGCAC-CGg -3' miRNA: 3'- acacuCGGUGUa----CCGUCUGCGUGaGC- -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 38131 | 0.69 | 0.519585 |
Target: 5'- ----cGUCGCAUGGUGGACGCGCa-- -3' miRNA: 3'- acacuCGGUGUACCGUCUGCGUGagc -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 44532 | 0.69 | 0.530195 |
Target: 5'- -uUGAGCgGCAcUGGCGGGUGCAC-CGg -3' miRNA: 3'- acACUCGgUGU-ACCGUCUGCGUGaGC- -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 26085 | 0.69 | 0.519585 |
Target: 5'- cGUGcaGGgCGCGauuaaccuugcGGCGGGCGCACUCGa -3' miRNA: 3'- aCAC--UCgGUGUa----------CCGUCUGCGUGAGC- -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 2034 | 0.76 | 0.203457 |
Target: 5'- gGUGAGCCGCAggggacUGGCGacgagaaguucGACGCACUgGu -3' miRNA: 3'- aCACUCGGUGU------ACCGU-----------CUGCGUGAgC- -5' |
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22720 | 3' | -55.5 | NC_005091.1 | + | 44272 | 1.1 | 0.000783 |
Target: 5'- aUGUGAGCCACAUGGCAGACGCACUCGg -3' miRNA: 3'- -ACACUCGGUGUACCGUCUGCGUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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