Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22722 | 3' | -54.9 | NC_005091.1 | + | 55055 | 0.66 | 0.749433 |
Target: 5'- gCCGGAcgaGGGGCgcagaaaacGGGAcAGCCGcGCgGCGCu -3' miRNA: 3'- -GGCCU---UCUCG---------UCCU-UUGGCuCG-CGUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 6270 | 0.66 | 0.759726 |
Target: 5'- gUGGuGGuGCGGGuuGGCCGGGUGCu- -3' miRNA: 3'- gGCCuUCuCGUCCu-UUGGCUCGCGug -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 11491 | 0.66 | 0.769889 |
Target: 5'- gCCGcGAaacaGGGGCAGG---CCGAaGCGCAg -3' miRNA: 3'- -GGC-CU----UCUCGUCCuuuGGCU-CGCGUg -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 57788 | 0.66 | 0.769889 |
Target: 5'- gCGGccAAGcGGCGGGGA---GAGCGCGCg -3' miRNA: 3'- gGCC--UUC-UCGUCCUUuggCUCGCGUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 394 | 0.66 | 0.769889 |
Target: 5'- gCGGccAAGcGGCGGGGA---GAGCGCGCg -3' miRNA: 3'- gGCC--UUC-UCGUCCUUuggCUCGCGUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 15167 | 0.66 | 0.728503 |
Target: 5'- cCCGGAucGGGGac-GAGACCGGccGCGUGCg -3' miRNA: 3'- -GGCCU--UCUCgucCUUUGGCU--CGCGUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 10411 | 0.66 | 0.739022 |
Target: 5'- aCGGAAGA-CAGGGgcuGACCguGAGCGgcCGCa -3' miRNA: 3'- gGCCUUCUcGUCCU---UUGG--CUCGC--GUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 46198 | 0.67 | 0.663772 |
Target: 5'- uCCGGcacGAGCAGGAGuUUG-GCGUGCa -3' miRNA: 3'- -GGCCuu-CUCGUCCUUuGGCuCGCGUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 28327 | 0.67 | 0.671423 |
Target: 5'- gCCGGuu--GCAccGGAAaccaucaucgucacGCCGGGCGCAUg -3' miRNA: 3'- -GGCCuucuCGU--CCUU--------------UGGCUCGCGUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 56426 | 0.67 | 0.674697 |
Target: 5'- gCGGGAcGGCAGGAAAUCuGGCGgGg -3' miRNA: 3'- gGCCUUcUCGUCCUUUGGcUCGCgUg -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 44829 | 0.67 | 0.700734 |
Target: 5'- gCCGGcAAGGGCGGcggcaaggcagcaacGAAGCCGAa-GCACu -3' miRNA: 3'- -GGCC-UUCUCGUC---------------CUUUGGCUcgCGUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 53761 | 0.67 | 0.717888 |
Target: 5'- aCGGuuugcGAGCAuu--GCCGAGCGCAa -3' miRNA: 3'- gGCCuu---CUCGUccuuUGGCUCGCGUg -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 55830 | 0.68 | 0.641843 |
Target: 5'- gCGacGAAGAGCGGcGcAGCCGgucagcaacuGGCGCACg -3' miRNA: 3'- gGC--CUUCUCGUC-CuUUGGC----------UCGCGUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 8086 | 0.68 | 0.61988 |
Target: 5'- gCGGccGAuGUAGGAAcgGCCGAcGUGCAUg -3' miRNA: 3'- gGCCuuCU-CGUCCUU--UGGCU-CGCGUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 7497 | 0.68 | 0.641843 |
Target: 5'- gCCGGuucuucGGGCAGcucgaccAAGCCGAgGCGCACc -3' miRNA: 3'- -GGCCuu----CUCGUCc------UUUGGCU-CGCGUG- -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 10294 | 0.69 | 0.565314 |
Target: 5'- aCGaAAGAGCuGGAAAUCG-GCGCGa -3' miRNA: 3'- gGCcUUCUCGuCCUUUGGCuCGCGUg -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 31780 | 0.69 | 0.565314 |
Target: 5'- aCGGcacGAGCAGGccGCCGAcgaGCGCGa -3' miRNA: 3'- gGCCuu-CUCGUCCuuUGGCU---CGCGUg -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 24799 | 0.69 | 0.587036 |
Target: 5'- aCCGGGauGGAGCAGGccaAAACaCucGCGCAg -3' miRNA: 3'- -GGCCU--UCUCGUCC---UUUG-GcuCGCGUg -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 56364 | 0.69 | 0.543806 |
Target: 5'- cCCGGAuuuacAGCGGGAGACCGAuuauucggccgGCGUc- -3' miRNA: 3'- -GGCCUuc---UCGUCCUUUGGCU-----------CGCGug -5' |
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22722 | 3' | -54.9 | NC_005091.1 | + | 6183 | 0.7 | 0.533152 |
Target: 5'- gCGGggGcuuGCGGGcgacGAGCCGAaGCgGCGCg -3' miRNA: 3'- gGCCuuCu--CGUCC----UUUGGCU-CG-CGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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