Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22722 | 5' | -57.2 | NC_005091.1 | + | 36727 | 0.66 | 0.626329 |
Target: 5'- cGGCUCGCCGuCGccAUCaACGUcgUCCGg -3' miRNA: 3'- uUCGAGCGGC-GC--UAGaUGCGaaGGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 40270 | 0.66 | 0.625237 |
Target: 5'- cGGCUCGCCGCagacguacgagaaGAUCg--GUUUCUCGu -3' miRNA: 3'- uUCGAGCGGCG-------------CUAGaugCGAAGGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 25526 | 0.66 | 0.61542 |
Target: 5'- gAGGCUCGCauguacaaCGCGuUCUACGagUUCCGg -3' miRNA: 3'- -UUCGAGCG--------GCGCuAGAUGCgaAGGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 11385 | 0.66 | 0.604527 |
Target: 5'- cGGGCgUCgGCCGCGucacgCUGCGCUUCggCCu -3' miRNA: 3'- -UUCG-AG-CGGCGCua---GAUGCGAAG--GGc -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 17958 | 0.66 | 0.604527 |
Target: 5'- cAGUugUCGCCGgcCGAUCU-CGCacaggUCCCGg -3' miRNA: 3'- uUCG--AGCGGC--GCUAGAuGCGa----AGGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 24388 | 0.66 | 0.593656 |
Target: 5'- cAGCcuaUCGCCGCGAUCaagGCGCagaUCGa -3' miRNA: 3'- uUCG---AGCGGCGCUAGa--UGCGaagGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 3508 | 0.66 | 0.582817 |
Target: 5'- cGGCUCGCUucauucgcaagGCG-UCU-CGCUguUCCCGa -3' miRNA: 3'- uUCGAGCGG-----------CGCuAGAuGCGA--AGGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 54947 | 0.66 | 0.572019 |
Target: 5'- -cGCUCGCCaaGcgCcGCGCggcugUCCCGu -3' miRNA: 3'- uuCGAGCGGcgCuaGaUGCGa----AGGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 16913 | 0.67 | 0.550576 |
Target: 5'- cAGCUUGgCGUGAUCgacaaUACGCUgacggUCCCu -3' miRNA: 3'- uUCGAGCgGCGCUAG-----AUGCGA-----AGGGc -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 51679 | 0.67 | 0.529385 |
Target: 5'- -cGCgcccgUGCCGCGcuUCUGCGCg-CCCGu -3' miRNA: 3'- uuCGa----GCGGCGCu-AGAUGCGaaGGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 41277 | 0.68 | 0.498189 |
Target: 5'- cAGCUUGCgGCGAUCUGC-CaUCgCGg -3' miRNA: 3'- uUCGAGCGgCGCUAGAUGcGaAGgGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 26054 | 0.68 | 0.487972 |
Target: 5'- cAGCUCGCCGCGcac-GCGCUgauaCCa -3' miRNA: 3'- uUCGAGCGGCGCuagaUGCGAag--GGc -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 35071 | 0.69 | 0.438476 |
Target: 5'- -cGC-CGCCGUGAauUCUACGCgUUCgCCa -3' miRNA: 3'- uuCGaGCGGCGCU--AGAUGCG-AAG-GGc -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 44136 | 0.7 | 0.395578 |
Target: 5'- gGAGUaCGUCGCGcaGUCUGucgaaugggcgcaguCGCUUCCCGg -3' miRNA: 3'- -UUCGaGCGGCGC--UAGAU---------------GCGAAGGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 40873 | 0.7 | 0.377791 |
Target: 5'- cGGuCUCGCCggaugcgugcgcagcGCGAUCUACGCU-CCgGa -3' miRNA: 3'- uUC-GAGCGG---------------CGCUAGAUGCGAaGGgC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 12435 | 0.71 | 0.340594 |
Target: 5'- -cGCUCGUCGCGGUCUuuuuguauagcCGCUucuUCCUGa -3' miRNA: 3'- uuCGAGCGGCGCUAGAu----------GCGA---AGGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 23261 | 0.71 | 0.309142 |
Target: 5'- cAGCUCGCCGaCGcaCUGCGCgacaugccgCCCGg -3' miRNA: 3'- uUCGAGCGGC-GCuaGAUGCGaa-------GGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 52972 | 0.77 | 0.122724 |
Target: 5'- -uGCUCGCCGCGAUCaGCGaacCCCGc -3' miRNA: 3'- uuCGAGCGGCGCUAGaUGCgaaGGGC- -5' |
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22722 | 5' | -57.2 | NC_005091.1 | + | 45152 | 1.06 | 0.001103 |
Target: 5'- gAAGCUCGCCGCGAUCUACGCUUCCCGc -3' miRNA: 3'- -UUCGAGCGGCGCUAGAUGCGAAGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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