Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22723 | 3' | -52 | NC_005091.1 | + | 51594 | 0.69 | 0.69173 |
Target: 5'- cGGGCGCGCAGaAGcGCGgcacgGGCGCGAu -3' miRNA: 3'- -CUUGUGCGUCcUCaUGUa----CUGCGCUu -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 50370 | 0.68 | 0.778479 |
Target: 5'- cGAACGCGCGGGccgccaaGCGUcGCGCGAu -3' miRNA: 3'- -CUUGUGCGUCCuca----UGUAcUGCGCUu -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 46657 | 1.06 | 0.002999 |
Target: 5'- uGAACACGCAGGAGUACAUGACGCGAAg -3' miRNA: 3'- -CUUGUGCGUCCUCAUGUACUGCGCUU- -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 43138 | 0.66 | 0.837108 |
Target: 5'- aGACACGCGGGAGaagcugggGCAaGugGcCGAu -3' miRNA: 3'- cUUGUGCGUCCUCa-------UGUaCugC-GCUu -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 40029 | 0.77 | 0.289223 |
Target: 5'- -uGCAUGUccugaAGGAGUGCAUGAcCGCGAAg -3' miRNA: 3'- cuUGUGCG-----UCCUCAUGUACU-GCGCUU- -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 37237 | 0.69 | 0.70292 |
Target: 5'- uGAACACGCcGGGcgcGUACGUGG-GCGAu -3' miRNA: 3'- -CUUGUGCGuCCU---CAUGUACUgCGCUu -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 35947 | 0.69 | 0.725066 |
Target: 5'- aGACugGCAGGaAGUGCGU-ACGCa-- -3' miRNA: 3'- cUUGugCGUCC-UCAUGUAcUGCGcuu -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 33602 | 0.66 | 0.854924 |
Target: 5'- aGGAaACGCAGGcAGc-CGUGAUGCGGAu -3' miRNA: 3'- -CUUgUGCGUCC-UCauGUACUGCGCUU- -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 32317 | 0.66 | 0.837108 |
Target: 5'- uGAACACGCGaGAuguccGUugAcGACGCGAAg -3' miRNA: 3'- -CUUGUGCGUcCU-----CAugUaCUGCGCUU- -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 2036 | 0.67 | 0.788725 |
Target: 5'- uGAGC-CGCAGGGG-AC-UGGCGaCGAGa -3' miRNA: 3'- -CUUGuGCGUCCUCaUGuACUGC-GCUU- -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 1197 | 0.76 | 0.32868 |
Target: 5'- --cCACGCAGGAGaUGCGUGucgauCGCGAGc -3' miRNA: 3'- cuuGUGCGUCCUC-AUGUACu----GCGCUU- -5' |
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22723 | 3' | -52 | NC_005091.1 | + | 1119 | 0.66 | 0.837108 |
Target: 5'- cGACACGCAucuccUGCGUGGCGUGAAg -3' miRNA: 3'- cUUGUGCGUccuc-AUGUACUGCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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