miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22724 3' -54.9 NC_005091.1 + 8134 0.68 0.590739
Target:  5'- gAGCCCggcaucgagauuacGCGCUACUggccgucauaucGCGGGGACCcGUa -3'
miRNA:   3'- -UUGGG--------------CGUGAUGA------------UGCCCUUGGaCAa -5'
22724 3' -54.9 NC_005091.1 + 8914 0.7 0.475114
Target:  5'- aGACCCGUACgaaGCUGCGGccGAgcACCUGa- -3'
miRNA:   3'- -UUGGGCGUGa--UGAUGCC--CU--UGGACaa -5'
22724 3' -54.9 NC_005091.1 + 47446 1.03 0.002573
Target:  5'- gAACCCGCACUACUACGGGAACCUGUUu -3'
miRNA:   3'- -UUGGGCGUGAUGAUGCCCUUGGACAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.