Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22725 | 5' | -61.5 | NC_005091.1 | + | 2307 | 0.66 | 0.414817 |
Target: 5'- cGGGCUUgGCGUCUUCCaCGUCGagCGu -3' miRNA: 3'- cCCCGAGaCGCGGGAGG-GCGGUaaGC- -5' |
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22725 | 5' | -61.5 | NC_005091.1 | + | 54965 | 0.66 | 0.39715 |
Target: 5'- -cGGCugucccguuuUCUGCGCCCcucgUCCgGCCGggCGg -3' miRNA: 3'- ccCCG----------AGACGCGGG----AGGgCGGUaaGC- -5' |
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22725 | 5' | -61.5 | NC_005091.1 | + | 14537 | 0.67 | 0.339263 |
Target: 5'- cGGGGCgacggCUGCuugcaacuggacGCCgaccaUUCCUGCCGUUUGa -3' miRNA: 3'- -CCCCGa----GACG------------CGG-----GAGGGCGGUAAGC- -5' |
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22725 | 5' | -61.5 | NC_005091.1 | + | 48496 | 1.09 | 0.000262 |
Target: 5'- cGGGGCUCUGCGCCCUCCCGCCAUUCGc -3' miRNA: 3'- -CCCCGAGACGCGGGAGGGCGGUAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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