Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22726 | 5' | -57.9 | NC_005091.1 | + | 23225 | 0.67 | 0.501515 |
Target: 5'- aCCGUGcCGcUGUGGuaGCGCgGCGCGu -3' miRNA: 3'- cGGUAC-GCaACGCCcgUGCGgUGUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 26507 | 0.67 | 0.52202 |
Target: 5'- gGCCAUGgacuuCGUcaggcuggccgUGCGGGCAuuggcCGCCuGCACa -3' miRNA: 3'- -CGGUAC-----GCA-----------ACGCCCGU-----GCGG-UGUGc -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 29607 | 0.67 | 0.54704 |
Target: 5'- cGCCGaucuCGgUGCGGGCgagcgggagggccguACGCUGCGCGg -3' miRNA: 3'- -CGGUac--GCaACGCCCG---------------UGCGGUGUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 33422 | 0.66 | 0.574557 |
Target: 5'- aGCaggGCGUccucGCGaGCGCGCUGCGCGa -3' miRNA: 3'- -CGguaCGCAa---CGCcCGUGCGGUGUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 48743 | 1.09 | 0.000564 |
Target: 5'- gGCCAUGCGUUGCGGGCACGCCACACGc -3' miRNA: 3'- -CGGUACGCAACGCCCGUGCGGUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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