miRNA: 3'- -CGGUaCGCAACGcc-CGugCGGuGUGC- -5'
miRNA: 3'- -CGGUAc--GCaAC-GCCCGUGCGgUGUGC- -5'
miRNA: 3'- cgGUACGCa---ACGCCCGUGCGG---UGUGc -5'
miRNA: 3'- -CGGUACGCA----AC-GCCCGUGCGg---UGUGC- -5'
miRNA: 3'- -CGGUACGCAACGCCCGUGCGGUGUGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22726 | 5' | -57.9 | NC_005091.1 | + | 50862 | 0.7 | 0.344993 |
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Target: 5'- cGCCGaGCGUUGCcaaGCugGCC-CGCGa -3' miRNA: 3'- -CGGUaCGCAACGcc-CGugCGGuGUGC- -5' |
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| 22726 | 5' | -57.9 | NC_005091.1 | + | 10438 | 0.72 | 0.257482 |
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Target: 5'- cGCCGUccuCGaUGuCGGGCGCGCgACACGc -3' miRNA: 3'- -CGGUAc--GCaAC-GCCCGUGCGgUGUGC- -5' |
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| 22726 | 5' | -57.9 | NC_005091.1 | + | 49032 | 0.77 | 0.119539 |
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Target: 5'- -aCGUGCGgugcUGCGGGCGCGCUgcuGCGCa -3' miRNA: 3'- cgGUACGCa---ACGCCCGUGCGG---UGUGc -5' |
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| 22726 | 5' | -57.9 | NC_005091.1 | + | 51577 | 0.79 | 0.093175 |
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Target: 5'- uGUCGUGCGUcgcgUGaCGGGCGCGCagaaGCGCGg -3' miRNA: 3'- -CGGUACGCA----AC-GCCCGUGCGg---UGUGC- -5' |
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| 22726 | 5' | -57.9 | NC_005091.1 | + | 48743 | 1.09 | 0.000564 |
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Target: 5'- gGCCAUGCGUUGCGGGCACGCCACACGc -3' miRNA: 3'- -CGGUACGCAACGCCCGUGCGGUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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