Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22727 | 3' | -55.7 | NC_005091.1 | + | 49183 | 0.94 | 0.013075 |
Target: 5'- uACCAGuaCCCACCGAUGCGGAUGACGu -3' miRNA: 3'- -UGGUCuaGGGUGGCUACGCCUACUGC- -5' |
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22727 | 3' | -55.7 | NC_005091.1 | + | 18459 | 0.67 | 0.64107 |
Target: 5'- cGCCGGGUCgUAguCCGA-GCGGAUcaGACGc -3' miRNA: 3'- -UGGUCUAGgGU--GGCUaCGCCUA--CUGC- -5' |
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22727 | 3' | -55.7 | NC_005091.1 | + | 32105 | 0.67 | 0.651959 |
Target: 5'- gGCCGGGUCCgCGCCGAcuuccggguugcUGCuGGAguccaucgccuUGugGa -3' miRNA: 3'- -UGGUCUAGG-GUGGCU------------ACG-CCU-----------ACugC- -5' |
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22727 | 3' | -55.7 | NC_005091.1 | + | 14939 | 0.66 | 0.695235 |
Target: 5'- gACgAGA-CCCGCCGcAUGCgaaccaccGGAUGGCc -3' miRNA: 3'- -UGgUCUaGGGUGGC-UACG--------CCUACUGc -5' |
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22727 | 3' | -55.7 | NC_005091.1 | + | 49636 | 0.66 | 0.705931 |
Target: 5'- uGCCA-AUCCgAUCGAUGCGGccguGUGAUu -3' miRNA: 3'- -UGGUcUAGGgUGGCUACGCC----UACUGc -5' |
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22727 | 3' | -55.7 | NC_005091.1 | + | 32183 | 0.66 | 0.73755 |
Target: 5'- gGCCGGAUCggaaguaaCUuCCGGUGCGGcgGcaGCGa -3' miRNA: 3'- -UGGUCUAG--------GGuGGCUACGCCuaC--UGC- -5' |
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22727 | 3' | -55.7 | NC_005091.1 | + | 48607 | 0.66 | 0.73755 |
Target: 5'- gGCCgucgGGGUCCCAuCCGGcgagGCGcGaAUGGCGg -3' miRNA: 3'- -UGG----UCUAGGGU-GGCUa---CGC-C-UACUGC- -5' |
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22727 | 3' | -55.7 | NC_005091.1 | + | 14024 | 0.66 | 0.7271 |
Target: 5'- cCCGaGUCCgCACCGAaGUGGAUcGACu -3' miRNA: 3'- uGGUcUAGG-GUGGCUaCGCCUA-CUGc -5' |
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22727 | 3' | -55.7 | NC_005091.1 | + | 27393 | 0.7 | 0.470571 |
Target: 5'- gGCUuGAUCCCgaagagaACCGGuUGaCGGAUGGCGa -3' miRNA: 3'- -UGGuCUAGGG-------UGGCU-AC-GCCUACUGC- -5' |
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22727 | 3' | -55.7 | NC_005091.1 | + | 34879 | 0.71 | 0.423066 |
Target: 5'- cGCCGGAUgcccucaucaaCCCGCUGAaGCGGAUcgccGACGc -3' miRNA: 3'- -UGGUCUA-----------GGGUGGCUaCGCCUA----CUGC- -5' |
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22727 | 3' | -55.7 | NC_005091.1 | + | 17938 | 0.73 | 0.303874 |
Target: 5'- cGCaCAGGUCCCgGCCGAUaGCGGAacguuugucgccgUGGCGc -3' miRNA: 3'- -UG-GUCUAGGG-UGGCUA-CGCCU-------------ACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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