Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22728 | 3' | -60.1 | NC_005091.1 | + | 28252 | 0.72 | 0.188262 |
Target: 5'- gGUUUCCgGugcaacCGGCGACGCGCuGGCGGc -3' miRNA: 3'- aCGAAGGgU------GUCGCUGCGCG-CCGCUa -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 12377 | 0.7 | 0.25632 |
Target: 5'- cGCgg-CCAguGCGACG-GCGGCGAc -3' miRNA: 3'- aCGaagGGUguCGCUGCgCGCCGCUa -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 31537 | 0.67 | 0.394089 |
Target: 5'- uUGCUggcgCUCguuACAGCGACGaGCGuGCGGUa -3' miRNA: 3'- -ACGAa---GGG---UGUCGCUGCgCGC-CGCUA- -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 15981 | 0.67 | 0.394089 |
Target: 5'- cUGCUUgUCGCucuGCGAUGCGaCGGCu-- -3' miRNA: 3'- -ACGAAgGGUGu--CGCUGCGC-GCCGcua -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 26357 | 0.67 | 0.41208 |
Target: 5'- gGCagCCC-CGGCGGCcCGCGcGCGGUc -3' miRNA: 3'- aCGaaGGGuGUCGCUGcGCGC-CGCUA- -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 37796 | 0.67 | 0.41208 |
Target: 5'- cGCUUCCCGCA-CG-CGCGCaGCc-- -3' miRNA: 3'- aCGAAGGGUGUcGCuGCGCGcCGcua -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 6437 | 0.66 | 0.46896 |
Target: 5'- cGC-UCCCgaggGCAGCGuGCGCGCuGCGc- -3' miRNA: 3'- aCGaAGGG----UGUCGC-UGCGCGcCGCua -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 33200 | 0.66 | 0.46896 |
Target: 5'- cUGCUucaugUCCUGuacgaggcCGGCGuaAUGCGCGGCGAc -3' miRNA: 3'- -ACGA-----AGGGU--------GUCGC--UGCGCGCCGCUa -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 34761 | 0.66 | 0.46896 |
Target: 5'- gGCcaugUCCCgGCcGCG-CGCGuCGGCGAUc -3' miRNA: 3'- aCGa---AGGG-UGuCGCuGCGC-GCCGCUA- -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 51140 | 1.05 | 0.000642 |
Target: 5'- uUGCUUCCCACAGCGACGCGCGGCGAUu -3' miRNA: 3'- -ACGAAGGGUGUCGCUGCGCGCCGCUA- -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 23511 | 0.72 | 0.173931 |
Target: 5'- aUGCagaUCAUGGCGacGCGCGCGGCGAUg -3' miRNA: 3'- -ACGaagGGUGUCGC--UGCGCGCCGCUA- -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 15343 | 0.7 | 0.23761 |
Target: 5'- aGCg-CCCACGGaCGgccaacgguacACGCGCGGCGGc -3' miRNA: 3'- aCGaaGGGUGUC-GC-----------UGCGCGCCGCUa -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 42058 | 0.68 | 0.327405 |
Target: 5'- aGCgUgCCACGGCGACGUGCuGCu-- -3' miRNA: 3'- aCGaAgGGUGUCGCUGCGCGcCGcua -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 20351 | 0.68 | 0.335266 |
Target: 5'- gGCUaCUCgcaaaGCGGCGACGCGuCGGcCGAUu -3' miRNA: 3'- aCGAaGGG-----UGUCGCUGCGC-GCC-GCUA- -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 45791 | 0.68 | 0.343264 |
Target: 5'- aGUagCCgGC-GCGGCGCGCGGcCGAc -3' miRNA: 3'- aCGaaGGgUGuCGCUGCGCGCC-GCUa -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 24891 | 0.68 | 0.351399 |
Target: 5'- gGCUUCCU-CGGCGGCcuGCGCagcacGGCGGa -3' miRNA: 3'- aCGAAGGGuGUCGCUG--CGCG-----CCGCUa -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 34556 | 0.67 | 0.376614 |
Target: 5'- gUGCUggcgacugCCgAguGgGAcCGCGCGGCGAa -3' miRNA: 3'- -ACGAa-------GGgUguCgCU-GCGCGCCGCUa -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 26688 | 0.67 | 0.385286 |
Target: 5'- aGCUUgCUGCuGCGGCGgccgGCGGCGGc -3' miRNA: 3'- aCGAAgGGUGuCGCUGCg---CGCCGCUa -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 48600 | 0.67 | 0.394089 |
Target: 5'- gGggUCCCAucCGGCGAgGCGCgaauGGCGGg -3' miRNA: 3'- aCgaAGGGU--GUCGCUgCGCG----CCGCUa -5' |
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22728 | 3' | -60.1 | NC_005091.1 | + | 2625 | 0.67 | 0.41208 |
Target: 5'- gGUcgCCCACGuaguucggguuGCGcACGCGCGGCaGAa -3' miRNA: 3'- aCGaaGGGUGU-----------CGC-UGCGCGCCG-CUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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