Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22728 | 5' | -50.1 | NC_005091.1 | + | 47064 | 0.73 | 0.647611 |
Target: 5'- gCCAgUCGAUGAAGAUgccgCGCUCGCGGucaGCGg -3' miRNA: 3'- -GGU-AGUUACUUUUA----GCGGGUGCC---CGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 48628 | 0.76 | 0.471952 |
Target: 5'- ---cCGAUGAGGAUCGCUCG-GGGCGg -3' miRNA: 3'- gguaGUUACUUUUAGCGGGUgCCCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 51177 | 1.11 | 0.003166 |
Target: 5'- uCCAUCAAUGAAAAUCGCCCACGGGCGc -3' miRNA: 3'- -GGUAGUUACUUUUAGCGGGUGCCCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 51273 | 0.68 | 0.908106 |
Target: 5'- aUCGUCGccGucg--CGCCCGUGGGCGa -3' miRNA: 3'- -GGUAGUuaCuuuuaGCGGGUGCCCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 57358 | 0.68 | 0.886654 |
Target: 5'- aCgGUCGauGUGAAGAU-GCUCgaaGCGGGCGg -3' miRNA: 3'- -GgUAGU--UACUUUUAgCGGG---UGCCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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