Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22728 | 5' | -50.1 | NC_005091.1 | + | 15352 | 0.74 | 0.591292 |
Target: 5'- -uGUCGGUGAAg--CGCCCACGGaCGg -3' miRNA: 3'- ggUAGUUACUUuuaGCGGGUGCCcGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 11657 | 0.66 | 0.954429 |
Target: 5'- gCCGUCA--GGAAGUCGCCgGCcuucagcggcucgauGGcGCGg -3' miRNA: 3'- -GGUAGUuaCUUUUAGCGGgUG---------------CC-CGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 7952 | 0.76 | 0.492854 |
Target: 5'- gCCGUUAcgaggacugacAUGGAAGUCGCCauccaaGCGGGCu -3' miRNA: 3'- -GGUAGU-----------UACUUUUAGCGGg-----UGCCCGc -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 6598 | 0.74 | 0.602522 |
Target: 5'- -aGUCGAUGA---UCGCUCGCGcGGCGu -3' miRNA: 3'- ggUAGUUACUuuuAGCGGGUGC-CCGC- -5' |
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22728 | 5' | -50.1 | NC_005091.1 | + | 5198 | 0.68 | 0.891885 |
Target: 5'- gCCGUCAcgGAAGAaguacgggcCGCCCAUgucacgccccgcagGGGCa -3' miRNA: 3'- -GGUAGUuaCUUUUa--------GCGGGUG--------------CCCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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