Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22728 | 5' | -50.1 | NC_005091.1 | + | 34868 | 0.67 | 0.914689 |
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Target: 5'- cUCAUCAAcccgcUGAAgcgGAUCGCCgACGcGcGCGg -3' miRNA: 3'- -GGUAGUU-----ACUU---UUAGCGGgUGC-C-CGC- -5' |
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| 22728 | 5' | -50.1 | NC_005091.1 | + | 30407 | 0.66 | 0.943264 |
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Target: 5'- aCCcgCGAcggGAAGAUCGUUCAcgccgcacCGGGCa -3' miRNA: 3'- -GGuaGUUa--CUUUUAGCGGGU--------GCCCGc -5' |
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| 22728 | 5' | -50.1 | NC_005091.1 | + | 26448 | 0.66 | 0.948113 |
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Target: 5'- cCCGUCAG-GAc---CGCgCGCGGGCc -3' miRNA: 3'- -GGUAGUUaCUuuuaGCGgGUGCCCGc -5' |
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| 22728 | 5' | -50.1 | NC_005091.1 | + | 46913 | 0.66 | 0.95268 |
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Target: 5'- uCCGUUcauGAUGAAcgucUCGUCCuguuCGGGCa -3' miRNA: 3'- -GGUAG---UUACUUuu--AGCGGGu---GCCCGc -5' |
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| 22728 | 5' | -50.1 | NC_005091.1 | + | 19166 | 0.79 | 0.357231 |
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Target: 5'- cCCGUCGAUGAAcuGGUCGCgaucaCCGUGGGCGa -3' miRNA: 3'- -GGUAGUUACUU--UUAGCG-----GGUGCCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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