Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22728 | 5' | -50.1 | NC_005091.1 | + | 37135 | 0.68 | 0.886654 |
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Target: 5'- aCgGUCucguUGAAccAAUCGCCCACGuacgcgcccGGCGu -3' miRNA: 3'- -GgUAGuu--ACUU--UUAGCGGGUGC---------CCGC- -5' |
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| 22728 | 5' | -50.1 | NC_005091.1 | + | 38892 | 0.68 | 0.886654 |
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Target: 5'- gCGUCGcgGUGAGAAgaugcaggCGCUugauUACGGGCGa -3' miRNA: 3'- gGUAGU--UACUUUUa-------GCGG----GUGCCCGC- -5' |
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| 22728 | 5' | -50.1 | NC_005091.1 | + | 30530 | 0.67 | 0.920984 |
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Target: 5'- gCGUC-AUGAGcAUCGCCacggACGGGUc -3' miRNA: 3'- gGUAGuUACUUuUAGCGGg---UGCCCGc -5' |
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| 22728 | 5' | -50.1 | NC_005091.1 | + | 22315 | 0.67 | 0.937598 |
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Target: 5'- aCCGUCAG-----GUCGCUCGugucgucCGGGCGa -3' miRNA: 3'- -GGUAGUUacuuuUAGCGGGU-------GCCCGC- -5' |
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| 22728 | 5' | -50.1 | NC_005091.1 | + | 51177 | 1.11 | 0.003166 |
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Target: 5'- uCCAUCAAUGAAAAUCGCCCACGGGCGc -3' miRNA: 3'- -GGUAGUUACUUUUAGCGGGUGCCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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