Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22730 | 3' | -53.2 | NC_005091.1 | + | 6695 | 0.69 | 0.662321 |
Target: 5'- cGUC-CUuaaggccggccaACAcCGCUUGGCUGCGGucGCCg -3' miRNA: 3'- -CAGaGA------------UGUuGCGAGCCGAUGCU--CGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 21520 | 0.69 | 0.640102 |
Target: 5'- -gCUCUGCcGCGUcgccUCGGCUugGuccgggguguAGCCg -3' miRNA: 3'- caGAGAUGuUGCG----AGCCGAugC----------UCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 4813 | 0.71 | 0.573584 |
Target: 5'- uUCUCUGuucGCGCUCGGCgcgGCG-GCa -3' miRNA: 3'- cAGAGAUgu-UGCGAGCCGa--UGCuCGg -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 7398 | 0.71 | 0.562617 |
Target: 5'- --gUCUGguGCGcCUCGGCUugguCGAGCUg -3' miRNA: 3'- cagAGAUguUGC-GAGCCGAu---GCUCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 22651 | 0.71 | 0.562617 |
Target: 5'- ---aCUACGAgGUUCGGCUGauAGCCg -3' miRNA: 3'- cagaGAUGUUgCGAGCCGAUgcUCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 43869 | 0.71 | 0.551708 |
Target: 5'- -gCUCUAU-GCGCUCGGCgcgcucUACGAGUa -3' miRNA: 3'- caGAGAUGuUGCGAGCCG------AUGCUCGg -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 15990 | 0.72 | 0.47755 |
Target: 5'- -gCUCUGCGAUGCgaCGGCUACaucggucaGGCCg -3' miRNA: 3'- caGAGAUGUUGCGa-GCCGAUGc-------UCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 38963 | 0.73 | 0.427711 |
Target: 5'- uUCUCaccGCGACGC-CGGCUGcCGAGaCCu -3' miRNA: 3'- cAGAGa--UGUUGCGaGCCGAU-GCUC-GG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 21217 | 0.74 | 0.372172 |
Target: 5'- uUCUagaaUUGCAGCGCaggCGGUUGCGAGCa -3' miRNA: 3'- cAGA----GAUGUUGCGa--GCCGAUGCUCGg -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 10224 | 0.74 | 0.363405 |
Target: 5'- ----gUGCGGCGCUCGGC-GCGuGCCg -3' miRNA: 3'- cagagAUGUUGCGAGCCGaUGCuCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 6968 | 0.75 | 0.346304 |
Target: 5'- -gCUCUACAACGCcgaccCGGCgucauCGAGCUg -3' miRNA: 3'- caGAGAUGUUGCGa----GCCGau---GCUCGG- -5' |
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22730 | 3' | -53.2 | NC_005091.1 | + | 53331 | 1.13 | 0.000973 |
Target: 5'- uGUCUCUACAACGCUCGGCUACGAGCCg -3' miRNA: 3'- -CAGAGAUGUUGCGAGCCGAUGCUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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