Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22730 | 5' | -56.2 | NC_005091.1 | + | 53365 | 1.05 | 0.001226 |
Target: 5'- uGUGGCCGCCGCCUGAGCAGCAAAAAUc -3' miRNA: 3'- -CACCGGCGGCGGACUCGUCGUUUUUA- -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 26601 | 0.77 | 0.1402 |
Target: 5'- -cGGCCGCCGCCggccgccgcAGCAGCAAGc-- -3' miRNA: 3'- caCCGGCGGCGGac-------UCGUCGUUUuua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 4019 | 0.71 | 0.306114 |
Target: 5'- cGUGGCgGCCGCUUcgaGAGCAaGCGcGAAUg -3' miRNA: 3'- -CACCGgCGGCGGA---CUCGU-CGUuUUUA- -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 26224 | 0.71 | 0.33821 |
Target: 5'- -cGGCgGCagcuucgGCCUGAGCGGCAGcccGAAUa -3' miRNA: 3'- caCCGgCGg------CGGACUCGUCGUU---UUUA- -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 11498 | 0.71 | 0.33821 |
Target: 5'- cGUGGCCGCCGCgaaacagGGGCAGgcCGAAGc- -3' miRNA: 3'- -CACCGGCGGCGga-----CUCGUC--GUUUUua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 7098 | 0.69 | 0.400063 |
Target: 5'- -cGGCCGCCGCUgc--CGGCAAGAc- -3' miRNA: 3'- caCCGGCGGCGGacucGUCGUUUUua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 23617 | 0.69 | 0.428692 |
Target: 5'- --cGCUGCCGCCgauGGCAcGCAGAAAUg -3' miRNA: 3'- cacCGGCGGCGGac-UCGU-CGUUUUUA- -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 32028 | 0.69 | 0.437514 |
Target: 5'- -cGGcCCGUCGCCUuggcgagcgacgcGAGCAGCAc---- -3' miRNA: 3'- caCC-GGCGGCGGA-------------CUCGUCGUuuuua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 55065 | 0.68 | 0.458495 |
Target: 5'- -gGGCCGCCcgGCCggacgagGGGC-GCAGAAAa -3' miRNA: 3'- caCCGGCGG--CGGa------CUCGuCGUUUUUa -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 26557 | 0.67 | 0.510481 |
Target: 5'- aGUGGCCuugaagcgcaaGUCGCC-GAGCAGgCAGAGc- -3' miRNA: 3'- -CACCGG-----------CGGCGGaCUCGUC-GUUUUua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 52118 | 0.67 | 0.521179 |
Target: 5'- -cGGCCGUCGCCggcgcgGGGCGcguuGCGGAc-- -3' miRNA: 3'- caCCGGCGGCGGa-----CUCGU----CGUUUuua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 40547 | 0.67 | 0.521179 |
Target: 5'- -aGGUCGCCGCCgccGAGuCGGUAu---- -3' miRNA: 3'- caCCGGCGGCGGa--CUC-GUCGUuuuua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 25159 | 0.67 | 0.542827 |
Target: 5'- cGUGGCCGCCgaucgguugaacGCCgcgcuuGCGGCAAu--- -3' miRNA: 3'- -CACCGGCGG------------CGGacu---CGUCGUUuuua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 10897 | 0.67 | 0.542827 |
Target: 5'- -cGGCgGCCgGCCUGua-GGCAAAAAg -3' miRNA: 3'- caCCGgCGG-CGGACucgUCGUUUUUa -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 16115 | 0.66 | 0.57582 |
Target: 5'- uUGuGCCGCCgGCCggcGGCAGCAc---- -3' miRNA: 3'- cAC-CGGCGG-CGGac-UCGUCGUuuuua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 4087 | 0.66 | 0.586925 |
Target: 5'- --aGCCGCC-CCgaaGGGCGGCGAAAc- -3' miRNA: 3'- cacCGGCGGcGGa--CUCGUCGUUUUua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 37261 | 0.66 | 0.586925 |
Target: 5'- -aGGCCGCCGCCa-AGUAcCGAAAGc -3' miRNA: 3'- caCCGGCGGCGGacUCGUcGUUUUUa -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 53105 | 0.66 | 0.609244 |
Target: 5'- -cGGCUGCCGCgCUGGGU--CAAGAc- -3' miRNA: 3'- caCCGGCGGCG-GACUCGucGUUUUua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 16032 | 0.66 | 0.620439 |
Target: 5'- -cGuGCUGCCGCCggccGGCGGCAc---- -3' miRNA: 3'- caC-CGGCGGCGGac--UCGUCGUuuuua -5' |
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22730 | 5' | -56.2 | NC_005091.1 | + | 26923 | 0.66 | 0.631644 |
Target: 5'- -cGGCCuUCGCCUGuuugacuuGGUGGCGAGAGa -3' miRNA: 3'- caCCGGcGGCGGAC--------UCGUCGUUUUUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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