Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22732 | 3' | -58.3 | NC_005091.1 | + | 16685 | 0.66 | 0.580009 |
Target: 5'- aCCCGagcuucgGAGuGUaga-AGGCCCaCGCGCCg -3' miRNA: 3'- -GGGCa------UUC-CGagaaUCCGGG-GCGCGG- -5' |
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22732 | 3' | -58.3 | NC_005091.1 | + | 45688 | 0.66 | 0.601219 |
Target: 5'- uUCGggauGGCUCcUGuacgcgucGGCCgCGCGCCg -3' miRNA: 3'- gGGCauu-CCGAGaAU--------CCGGgGCGCGG- -5' |
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22732 | 3' | -58.3 | NC_005091.1 | + | 26320 | 0.66 | 0.601219 |
Target: 5'- gCUCGUAuucGGGCUgccgCUcAGGCCgaagcugccgCCGCGUCg -3' miRNA: 3'- -GGGCAU---UCCGA----GAaUCCGG----------GGCGCGG- -5' |
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22732 | 3' | -58.3 | NC_005091.1 | + | 9659 | 0.68 | 0.466711 |
Target: 5'- cUCUGgcAGGCUCUcgugucccggcUGGGCCagcgacucaucgaCCGgGCCu -3' miRNA: 3'- -GGGCauUCCGAGA-----------AUCCGG-------------GGCgCGG- -5' |
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22732 | 3' | -58.3 | NC_005091.1 | + | 7080 | 0.68 | 0.487361 |
Target: 5'- uCCUGaAGGGCaUCgagacGGCCgCCGCuGCCg -3' miRNA: 3'- -GGGCaUUCCG-AGaau--CCGG-GGCG-CGG- -5' |
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22732 | 3' | -58.3 | NC_005091.1 | + | 40508 | 0.68 | 0.448426 |
Target: 5'- aCCGcGAGGCguaccccgAGGUCCCGCaGCUc -3' miRNA: 3'- gGGCaUUCCGagaa----UCCGGGGCG-CGG- -5' |
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22732 | 3' | -58.3 | NC_005091.1 | + | 55229 | 0.68 | 0.448426 |
Target: 5'- cCCCGgucGGCUCguucgacgcGGCCCgUGCGCg -3' miRNA: 3'- -GGGCauuCCGAGaau------CCGGG-GCGCGg -5' |
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22732 | 3' | -58.3 | NC_005091.1 | + | 23415 | 0.72 | 0.256642 |
Target: 5'- -aCGUGGGGC-CUUGGcGCuUCCGUGCCu -3' miRNA: 3'- ggGCAUUCCGaGAAUC-CG-GGGCGCGG- -5' |
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22732 | 3' | -58.3 | NC_005091.1 | + | 53253 | 0.73 | 0.226272 |
Target: 5'- aCCGgAAGGCUCgc--GCCCgCGCGCUa -3' miRNA: 3'- gGGCaUUCCGAGaaucCGGG-GCGCGG- -5' |
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22732 | 3' | -58.3 | NC_005091.1 | + | 11025 | 0.75 | 0.165664 |
Target: 5'- gCCGUAAGGCaUCgcccuuuGGCCCCGacggguuucCGCCg -3' miRNA: 3'- gGGCAUUCCG-AGaau----CCGGGGC---------GCGG- -5' |
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22732 | 3' | -58.3 | NC_005091.1 | + | 54878 | 1.06 | 0.001066 |
Target: 5'- cCCCGUAAGGCUC-UAGGCCCCGCGCCg -3' miRNA: 3'- -GGGCAUUCCGAGaAUCCGGGGCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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