Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22732 | 5' | -53.6 | NC_005091.1 | + | 54913 | 1.09 | 0.001329 |
Target: 5'- cUAGGGGUAAACCCUAUGAUGCGCCGCc -3' miRNA: 3'- -AUCCCCAUUUGGGAUACUACGCGGCG- -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 47292 | 0.73 | 0.379917 |
Target: 5'- aGGGGGUAGACCCguacaacacGAUaaacacCGCCGCa -3' miRNA: 3'- aUCCCCAUUUGGGaua------CUAc-----GCGGCG- -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 41505 | 0.73 | 0.398272 |
Target: 5'- gUGGGGGUucuUCUUcGUGAUGCGuuGCg -3' miRNA: 3'- -AUCCCCAuuuGGGA-UACUACGCggCG- -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 4503 | 0.72 | 0.447553 |
Target: 5'- cGGGGGUucaaccgguugaagcGGCCC-AUGAacUGCGCCGUu -3' miRNA: 3'- aUCCCCAu--------------UUGGGaUACU--ACGCGGCG- -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 2651 | 0.69 | 0.640885 |
Target: 5'- -cGGGGaAGACCCguUGucgcagGUGCCGCu -3' miRNA: 3'- auCCCCaUUUGGGauACua----CGCGGCG- -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 48793 | 0.69 | 0.640885 |
Target: 5'- -cGGGGauucGGACCuagCUGUGAgacGCGCUGCg -3' miRNA: 3'- auCCCCa---UUUGG---GAUACUa--CGCGGCG- -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 9678 | 0.68 | 0.696614 |
Target: 5'- cGGGGGUAaaggauauugagAAUCaggAUGGUaGCGCCGUu -3' miRNA: 3'- aUCCCCAU------------UUGGga-UACUA-CGCGGCG- -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 55168 | 0.67 | 0.718532 |
Target: 5'- cGGGGGUuacuucacAACCCUAgccgGAcccccgGuCGCCGCc -3' miRNA: 3'- aUCCCCAu-------UUGGGAUa---CUa-----C-GCGGCG- -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 16232 | 0.67 | 0.729365 |
Target: 5'- cAGGGacgAGGCCgccggcaUGcGAUGCGCCGCu -3' miRNA: 3'- aUCCCca-UUUGGg------AUaCUACGCGGCG- -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 16626 | 0.66 | 0.791813 |
Target: 5'- cGGGGGcUGGACuCCUGU--UGCGaCCGg -3' miRNA: 3'- aUCCCC-AUUUG-GGAUAcuACGC-GGCg -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 28928 | 0.66 | 0.801685 |
Target: 5'- aAGGGGacaaGAACCCgaccgAUGAaGCGUauCGCc -3' miRNA: 3'- aUCCCCa---UUUGGGa----UACUaCGCG--GCG- -5' |
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22732 | 5' | -53.6 | NC_005091.1 | + | 10419 | 0.66 | 0.811373 |
Target: 5'- cAGGGGcuGACC--GUGA-GCGgCCGCa -3' miRNA: 3'- aUCCCCauUUGGgaUACUaCGC-GGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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