miRNA display CGI


Results 1 - 14 of 14 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22733 3' -51.2 NC_005091.1 + 32811 0.66 0.926989
Target:  5'- aGCGAUGgcguAGGCGGCCGAGuagcaguucGAGUCgAUGa -3'
miRNA:   3'- -CGUUGC----UCCGCUGGUUCc--------UUCAG-UAC- -5'
22733 3' -51.2 NC_005091.1 + 44845 0.66 0.914689
Target:  5'- gGCAACGAccacGGCGGCCGgcaAGGgcGgcggCAa- -3'
miRNA:   3'- -CGUUGCU----CCGCUGGU---UCCuuCa---GUac -5'
22733 3' -51.2 NC_005091.1 + 25074 0.66 0.894085
Target:  5'- cGCGGCGuucaaccgaucGGCGGCCAcGGAggcGGUCGc- -3'
miRNA:   3'- -CGUUGCu----------CCGCUGGUuCCU---UCAGUac -5'
22733 3' -51.2 NC_005091.1 + 48158 0.69 0.778421
Target:  5'- -gGACaAGGCGAgCCGGGGuucuaauGGUCGUGg -3'
miRNA:   3'- cgUUGcUCCGCU-GGUUCCu------UCAGUAC- -5'
22733 3' -51.2 NC_005091.1 + 52126 0.69 0.778421
Target:  5'- cGCGGCGcguaucucGCGAUUGAGGAAGUCAc- -3'
miRNA:   3'- -CGUUGCuc------CGCUGGUUCCUUCAGUac -5'
22733 3' -51.2 NC_005091.1 + 48617 0.69 0.778421
Target:  5'- cGCu-CGGGGCGGCCGucGggGUCc-- -3'
miRNA:   3'- -CGuuGCUCCGCUGGUucCuuCAGuac -5'
22733 3' -51.2 NC_005091.1 + 46032 0.7 0.747041
Target:  5'- cGCAGCGAGaGCGGC--GGGGAGaUCGa- -3'
miRNA:   3'- -CGUUGCUC-CGCUGguUCCUUC-AGUac -5'
22733 3' -51.2 NC_005091.1 + 10744 0.7 0.714558
Target:  5'- gGCGG-GAGGCGAUCAAGaAGGcCAUGg -3'
miRNA:   3'- -CGUUgCUCCGCUGGUUCcUUCaGUAC- -5'
22733 3' -51.2 NC_005091.1 + 24810 0.71 0.692456
Target:  5'- uGCuGCGcAGGCcGCCGAGGAAGcCGUu -3'
miRNA:   3'- -CGuUGC-UCCGcUGGUUCCUUCaGUAc -5'
22733 3' -51.2 NC_005091.1 + 1003 0.71 0.681307
Target:  5'- cGCGACGAacauGGCGugCGAGcAGGUCGc- -3'
miRNA:   3'- -CGUUGCU----CCGCugGUUCcUUCAGUac -5'
22733 3' -51.2 NC_005091.1 + 55609 0.72 0.636334
Target:  5'- --cGCGugacGGCGACCGucaucAGGAAGUCGUa -3'
miRNA:   3'- cguUGCu---CCGCUGGU-----UCCUUCAGUAc -5'
22733 3' -51.2 NC_005091.1 + 14423 0.72 0.591292
Target:  5'- uGCggUGAGaGCGACCGgcgucGGGAAGUugaaCGUGa -3'
miRNA:   3'- -CGuuGCUC-CGCUGGU-----UCCUUCA----GUAC- -5'
22733 3' -51.2 NC_005091.1 + 55272 0.75 0.431556
Target:  5'- uCAACGGcGGCGACCGGGGGuccggcuagGGUUGUGa -3'
miRNA:   3'- cGUUGCU-CCGCUGGUUCCU---------UCAGUAC- -5'
22733 3' -51.2 NC_005091.1 + 55383 1.12 0.001824
Target:  5'- gGCAACGAGGCGACCAAGGAAGUCAUGg -3'
miRNA:   3'- -CGUUGCUCCGCUGGUUCCUUCAGUAC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.