Results 21 - 40 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22734 | 3' | -67.2 | NC_005091.1 | + | 53597 | 0.68 | 0.176599 |
Target: 5'- gCGCCGuCCGAuuccaaauagaaCCcCuuGCCCGUGCUGu -3' miRNA: 3'- -GCGGCcGGCU------------GGaGggCGGGCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 9329 | 0.68 | 0.17398 |
Target: 5'- uCGCCGGCCGgucgcccuucgcaggGCCgcaCCGCcggaaacaCCGCGCg- -3' miRNA: 3'- -GCGGCCGGC---------------UGGag-GGCG--------GGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 49807 | 0.68 | 0.172253 |
Target: 5'- aCGCaCGGCCGACgUgcCCCGCaugcagagcuaCGCGCa- -3' miRNA: 3'- -GCG-GCCGGCUGgA--GGGCGg----------GCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 55907 | 0.68 | 0.168002 |
Target: 5'- gCGCagcaGGCCGACCaaCUGCgUGCGCa- -3' miRNA: 3'- -GCGg---CCGGCUGGagGGCGgGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 31538 | 0.68 | 0.159782 |
Target: 5'- uGCUGGCgUGAUUcagUCCgGCCCGgCGCUGu -3' miRNA: 3'- gCGGCCG-GCUGG---AGGgCGGGC-GCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 53423 | 0.69 | 0.14926 |
Target: 5'- uCGCUGGCCgugcauucgcccuuaGACCaUCCCGCcagaaagCCGCGCc- -3' miRNA: 3'- -GCGGCCGG---------------CUGG-AGGGCG-------GGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 36735 | 0.69 | 0.144423 |
Target: 5'- aCGCUGGCCGAaguCCagCCCGuCCCGUGa-- -3' miRNA: 3'- -GCGGCCGGCU---GGa-GGGC-GGGCGCgac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 39889 | 0.69 | 0.137259 |
Target: 5'- aCG-CGGCCGACCaUCCGgCUGCGCc- -3' miRNA: 3'- -GCgGCCGGCUGGaGGGCgGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 26524 | 0.69 | 0.13244 |
Target: 5'- gGCUGGCCGugcgggcauuggccGCCUgcacauccugaCCCGCCuucgcgaCGCGCUGg -3' miRNA: 3'- gCGGCCGGC--------------UGGA-----------GGGCGG-------GCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 31710 | 0.69 | 0.130423 |
Target: 5'- gCGCCGGCCG-CCUUCgGC-UGCGCc- -3' miRNA: 3'- -GCGGCCGGCuGGAGGgCGgGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 8097 | 0.69 | 0.127125 |
Target: 5'- uGCaGGCCGACCucgaUCCC-CUCGCGCa- -3' miRNA: 3'- gCGgCCGGCUGG----AGGGcGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 38952 | 0.69 | 0.126799 |
Target: 5'- aCGCCGGCUgccgaGACCUaugaacaCCUGUUCGCGCa- -3' miRNA: 3'- -GCGGCCGG-----CUGGA-------GGGCGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 40928 | 0.7 | 0.117686 |
Target: 5'- gCGUaCGGCCGAgguugugaaCCugaacaccgcgUCCCGCCCaGCGCUGc -3' miRNA: 3'- -GCG-GCCGGCU---------GG-----------AGGGCGGG-CGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 34757 | 0.71 | 0.095621 |
Target: 5'- uGUCGGCC-AUgUCCCGgCCGCGCg- -3' miRNA: 3'- gCGGCCGGcUGgAGGGCgGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 56269 | 0.71 | 0.095372 |
Target: 5'- aCGCCGGCCGAauaaucggUCUCCCGCuguaaauCCGgGCc- -3' miRNA: 3'- -GCGGCCGGCU--------GGAGGGCG-------GGCgCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 54989 | 0.72 | 0.088171 |
Target: 5'- uCGuCCGGCCGGgCggCCCGCcgguaucCCGUGCUGa -3' miRNA: 3'- -GC-GGCCGGCUgGa-GGGCG-------GGCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 17952 | 0.72 | 0.086114 |
Target: 5'- uCGCCGGCCGAUCucgcacaggUCCCGgCCGauaGCg- -3' miRNA: 3'- -GCGGCCGGCUGG---------AGGGCgGGCg--CGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 4313 | 0.72 | 0.077511 |
Target: 5'- gCGCCGGCCGucguCUUCCUGuuuCCCG-GCUGg -3' miRNA: 3'- -GCGGCCGGCu---GGAGGGC---GGGCgCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 30181 | 0.72 | 0.075492 |
Target: 5'- gGUCGG-CGACUUCgCCGCCCuuaaaGCGCUGg -3' miRNA: 3'- gCGGCCgGCUGGAG-GGCGGG-----CGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 33220 | 0.74 | 0.054889 |
Target: 5'- gGCCGGCguaaugcgcggCGACCaUCUGCCCGCGCa- -3' miRNA: 3'- gCGGCCG-----------GCUGGaGGGCGGGCGCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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