miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22734 5' -55 NC_005091.1 + 56049 1.1 0.00108
Target:  5'- gACCUUCCUGAGCAAGAUGCCGGUCGGc -3'
miRNA:   3'- -UGGAAGGACUCGUUCUACGGCCAGCC- -5'
22734 5' -55 NC_005091.1 + 17866 0.68 0.632794
Target:  5'- gGCCgggaCCUGuGCGAGAUcgGCCGG-CGa -3'
miRNA:   3'- -UGGaa--GGACuCGUUCUA--CGGCCaGCc -5'
22734 5' -55 NC_005091.1 + 22449 0.67 0.676585
Target:  5'- cGCCUUUC-GGGuCAucuGcgGCUGGUCGGu -3'
miRNA:   3'- -UGGAAGGaCUC-GUu--CuaCGGCCAGCC- -5'
22734 5' -55 NC_005091.1 + 43634 0.66 0.740788
Target:  5'- cACCUUUCUGccaAGCGAGAaaUGcCCGGaccuucacgaaaUCGGc -3'
miRNA:   3'- -UGGAAGGAC---UCGUUCU--AC-GGCC------------AGCC- -5'
22734 5' -55 NC_005091.1 + 7071 0.66 0.740788
Target:  5'- uGCCUUCCgccAGCAGcucGAUgacgcGCgGGUCGGc -3'
miRNA:   3'- -UGGAAGGac-UCGUU---CUA-----CGgCCAGCC- -5'
22734 5' -55 NC_005091.1 + 1243 0.65 0.77157
Target:  5'- gGCCgg-CUGGGCcGGAUGUCGGaaGGc -3'
miRNA:   3'- -UGGaagGACUCGuUCUACGGCCagCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.