Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22734 | 5' | -55 | NC_005091.1 | + | 56049 | 1.1 | 0.00108 |
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Target: 5'- gACCUUCCUGAGCAAGAUGCCGGUCGGc -3' miRNA: 3'- -UGGAAGGACUCGUUCUACGGCCAGCC- -5' |
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| 22734 | 5' | -55 | NC_005091.1 | + | 17866 | 0.68 | 0.632794 |
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Target: 5'- gGCCgggaCCUGuGCGAGAUcgGCCGG-CGa -3' miRNA: 3'- -UGGaa--GGACuCGUUCUA--CGGCCaGCc -5' |
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| 22734 | 5' | -55 | NC_005091.1 | + | 22449 | 0.67 | 0.676585 |
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Target: 5'- cGCCUUUC-GGGuCAucuGcgGCUGGUCGGu -3' miRNA: 3'- -UGGAAGGaCUC-GUu--CuaCGGCCAGCC- -5' |
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| 22734 | 5' | -55 | NC_005091.1 | + | 43634 | 0.66 | 0.740788 |
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Target: 5'- cACCUUUCUGccaAGCGAGAaaUGcCCGGaccuucacgaaaUCGGc -3' miRNA: 3'- -UGGAAGGAC---UCGUUCU--AC-GGCC------------AGCC- -5' |
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| 22734 | 5' | -55 | NC_005091.1 | + | 7071 | 0.66 | 0.740788 |
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Target: 5'- uGCCUUCCgccAGCAGcucGAUgacgcGCgGGUCGGc -3' miRNA: 3'- -UGGAAGGac-UCGUU---CUA-----CGgCCAGCC- -5' |
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| 22734 | 5' | -55 | NC_005091.1 | + | 1243 | 0.65 | 0.77157 |
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Target: 5'- gGCCgg-CUGGGCcGGAUGUCGGaaGGc -3' miRNA: 3'- -UGGaagGACUCGuUCUACGGCCagCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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